ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYGREDTTI EPDFIEPDAP LAASGGVADN IGGTMQNSGS RGTLDETVLQ TLKRDVVEIN
70 80 90 100 110 120
SRLKQVVYPH FPSFFSPSDD GIGAADNDIS ANCDLWAPLA FIILYSLFVS HARSLFSSLF
130 140 150 160 170 180
VSSWFILLVM ALHLRLTKPH QRVSLISYIS ISGYCLFPQV LNALVSQILL PLAYHIGKQN
190 200 210 220 230
RWIVRVLSLV KLVVMALCLM WSVAAVSWVT KSKTIIEIYP LALCLFGMAW LSTIL
References and
documentation are available.
Number of amino acids: 235
Molecular weight: 25995.45
Theoretical pI: 6.50
Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 1196
Hydrogen H 1875
Nitrogen N 299
Oxygen O 327
Sulfur S 10
Formula: C
1196H
1875N
299O
327S
10
Total number of atoms: 3707
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43680
Abs 0.1% (=1 g/l) 1.680, assuming all pairs of Cys residues form cystines
Ext. coefficient 43430
Abs 0.1% (=1 g/l) 1.671, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.69
This classifies the protein as unstable.
Aliphatic index: 118.64
Grand average of hydropathicity (GRAVY): 0.521