ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYKFITKNK KYTPMSSPGN SGVAIDSTVL KAIELGTRLF KSGEYLQAKR IFTNALRVCD
70 80 90 100 110 120
SYSQEQIMRI RNAYQLDTAR PDNKRLYHPR YIKILDNICA CYEKLNDLKS CLDVSQRLLK
130 140 150 160 170 180
LEPGNIKCYI RCTRTLIKLK DWKRAYKTCS RGLQLCNNDS NHLRQQKQFI KNNMVQKQDG
190 200 210 220 230 240
KRSYIDPLEE TKIAKKKKNN NVLESLPKKK IKGSTKKTDL VGNLPIEILP IIFQRFTTKE
250 260 270 280 290 300
LVTLSLVCNK WRDKILYHLD CFQEFNLAPI NFKNFVKFMD FLQQNFTRTY RKYILSQVKV
310 320 330 340 350 360
SSRITSEELR ITQLLFSKMP KCINIERLIL SMPTLTTTQI FKLMVRGGTD FFTRLLELSL
370 380 390 400 410 420
MITYRPDKQH ELEILQTCPL LKKIELIFVN SLVPIFDGNN SVGRDGSFNV MARHTNMQIS
430 440 450 460 470 480
TADNDEQGIV EEKVIYSELE KITLICDKKK IKNFPLCRAL LRGQFPLLQK LTITGVTFPM
490 500 510 520 530 540
NNQDIMNFQW LLNFPDLKEL WIEDNDNCEL SKFLQLLKFS NVWKNLEKLT FRENKLYPIV
550 560 570 580 590 600
NLDEDQPVTN DDEVPSMLFY KENLQNLEKL DLMGTSISGS ALTRLCEQEY LDGRKLRSLN
610 620 630 640 650 660
IGNCPNIQFP NNHAHTARMI LDVNAVLKRL SKLEEINLSH LSSLNDSTMK SFIINVPFLE
670 680 690 700 710 720
NLKRLDISHN FEITGISIYE FLKKFQMDHD NEAGGQPLAY LNIDGCSQVS HITVNMIRAQ
730 740
NLVTQVDCVY ERDVWRKFGI NSYSYS
References and
documentation are available.
Number of amino acids: 746
Molecular weight: 86800.88
Theoretical pI: 9.22
Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 107
Atomic composition:
Carbon C 3884
Hydrogen H 6232
Nitrogen N 1054
Oxygen O 1118
Sulfur S 38
Formula: C
3884H
6232N
1054O
1118S
38
Total number of atoms: 12326
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 69885
Abs 0.1% (=1 g/l) 0.805, assuming all pairs of Cys residues form cystines
Ext. coefficient 68760
Abs 0.1% (=1 g/l) 0.792, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.73
This classifies the protein as stable.
Aliphatic index: 95.50
Grand average of hydropathicity (GRAVY): -0.357