ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG TTGNQLYMGD 

        70         80         90        100        110        120 
LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM GPKNNQGYCF VDFPSSTHAA 

       130        140        150        160        170        180 
NALLKNGMLI PNFPNKKLKL NWATSSYSNS NNSLNNVKSG NNCSIFVGDL APNVTESQLF 

       190        200        210        220        230        240 
ELFINRYAST SHAKIVHDQV TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT 

       250        260        270        280        290        300 
SGQQQHVSGN NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ 

       310        320        330        340        350        360 
QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC GFVQYVDRLS 

       370        380        390        400        410        420 
AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS LQVQQQQPGL QQPNYGYIPS 

       430        440        450        460        470        480 
STCEAPVLPD NNVSSTMLPG CQILNYSNPY ANANGLGSNN FSFYSNNNAT NTQATSLLAD 

       490        500        510        520 
TSSMDLSGTG GQQVIMQGSE AVVNSTNAML NRLEQGSNGF MFA 

References and documentation are available.

Number of amino acids: 523

Molecular weight: 56972.21

Theoretical pI: 8.59

Amino acid composition: 
Ala (A)  30	  5.7%
Arg (R)  16	  3.1%
Asn (N)  65	 12.4%
Asp (D)  16	  3.1%
Cys (C)   6	  1.1%
Gln (Q)  37	  7.1%
Glu (E)  13	  2.5%
Gly (G)  42	  8.0%
His (H)   6	  1.1%
Ile (I)  17	  3.3%
Leu (L)  43	  8.2%
Lys (K)  17	  3.3%
Met (M)  17	  3.3%
Phe (F)  23	  4.4%
Pro (P)  22	  4.2%
Ser (S)  66	 12.6%
Thr (T)  32	  6.1%
Trp (W)   5	  1.0%
Tyr (Y)  19	  3.6%
Val (V)  31	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      2469
Hydrogen    H	      3831
Nitrogen    N	       707
Oxygen      O	       801
Sulfur      S	        23

Formula: C2469H3831N707O801S23
Total number of atoms: 7831

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56185
Abs 0.1% (=1 g/l)   0.986, assuming all pairs of Cys residues form cystines


Ext. coefficient    55810
Abs 0.1% (=1 g/l)   0.980, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.45
This classifies the protein as stable.



Aliphatic index: 67.67

Grand average of hydropathicity (GRAVY): -0.452