ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV 

        70         80         90        100        110        120 
QKQIETHMEK REIEVQVSEL RDRLEEEETL SEEQIDKKCE ALRAKLTNEW QEQQRMSSLY 

       130 
TPRKARLTEE QHRHE 

References and documentation are available.

Number of amino acids: 135

Molecular weight: 15762.54

Theoretical pI: 9.67

Amino acid composition: 
Ala (A)   9	  6.7%
Arg (R)  15	 11.1%
Asn (N)   5	  3.7%
Asp (D)   3	  2.2%
Cys (C)   1	  0.7%
Gln (Q)  16	 11.9%
Glu (E)  17	 12.6%
Gly (G)   5	  3.7%
His (H)   5	  3.7%
Ile (I)   5	  3.7%
Leu (L)  10	  7.4%
Lys (K)  11	  8.1%
Met (M)   3	  2.2%
Phe (F)   0	  0.0%
Pro (P)   3	  2.2%
Ser (S)  14	 10.4%
Thr (T)   6	  4.4%
Trp (W)   1	  0.7%
Tyr (Y)   2	  1.5%
Val (V)   4	  3.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	       658
Hydrogen    H	      1095
Nitrogen    N	       223
Oxygen      O	       219
Sulfur      S	         4

Formula: C658H1095N223O219S4
Total number of atoms: 2199

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.538, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.538, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 75.12
This classifies the protein as unstable.



Aliphatic index: 58.59

Grand average of hydropathicity (GRAVY): -1.436