ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYNHLFKIV VVGDHNCGKS CILLRFAENS FRMDHISTLG VDFKLKTIKL GRDKIRLELW 

        70         80         90        100        110        120 
DTAGMERYRT IYNSYYHSAH GVMCVYDMTN EKSFENLEKY WLKEIKQHAP PNAVLMLVGN 

       130        140        150        160        170        180 
KADMDQERKV DFDRAEKLAS RLGVSLYEVS AKTGINCEEA FHTLTAAMRE RITAGSMHSD 

       190        200        210 
ESDDNEEFSS AFHVDGVIKS DKGKFTSCCA SAPEPAFV 

References and documentation are available.

Number of amino acids: 218

Molecular weight: 24754.13

Theoretical pI: 6.14

Amino acid composition: 
Ala (A)  17	  7.8%
Arg (R)  11	  5.0%
Asn (N)  10	  4.6%
Asp (D)  15	  6.9%
Cys (C)   6	  2.8%
Gln (Q)   2	  0.9%
Glu (E)  17	  7.8%
Gly (G)  12	  5.5%
His (H)   9	  4.1%
Ile (I)  10	  4.6%
Leu (L)  16	  7.3%
Lys (K)  17	  7.8%
Met (M)   9	  4.1%
Phe (F)  11	  5.0%
Pro (P)   4	  1.8%
Ser (S)  18	  8.3%
Thr (T)  10	  4.6%
Trp (W)   2	  0.9%
Tyr (Y)   8	  3.7%
Val (V)  14	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1088
Hydrogen    H	      1694
Nitrogen    N	       300
Oxygen      O	       331
Sulfur      S	        15

Formula: C1088H1694N300O331S15
Total number of atoms: 3428

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23295
Abs 0.1% (=1 g/l)   0.941, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.926, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 22.28
This classifies the protein as stable.



Aliphatic index: 72.94

Grand average of hydropathicity (GRAVY): -0.391