ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYNNPYQLE TPFEESYELD EGSSAIGAEG HDFVGFMNKI SQINRDLDKY DHTINQVDSL
70 80 90 100 110 120
HKRLLTEVNE EQASHLRHSL DNFVAQATDL QFKLKNEIKS AQRDGIHDTN KQAQAENSRQ
130 140 150 160 170 180
RFLKLIQDYR IVDSNYKEEN KEQAKRQYMI IQPEATEDEV EAAISDVGGQ QIFSQALLNA
190 200 210 220 230 240
NRRGEAKTAL AEVQARHQEL LKLEKSMAEL TQLFNDMEEL VIEQQENVDV IDKNVEDAQL
250 260 270 280 290
DVEQGVGHTD KAVKSARKAR KNKIRCWLIV FAIIVVVVVV VVVPAVVKTR
References and
documentation are available.
Number of amino acids: 290
Molecular weight: 33106.17
Theoretical pI: 5.17
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1445
Hydrogen H 2319
Nitrogen N 415
Oxygen O 463
Sulfur S 6
Formula: C
1445H
2319N
415O
463S
6
Total number of atoms: 4648
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.481, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.481, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.86
This classifies the protein as stable.
Aliphatic index: 90.76
Grand average of hydropathicity (GRAVY): -0.582