ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYNYLKAAR KIICIGRNYA AHIKELNNST PKQPFFFLKP TSSIVTPLSS SLVKTTRPAN 

        70         80         90        100        110        120 
STFNGLNEDG TNPGPIFIPR GVKVHHEIEL ALIVSKHLSN VTKMKPEEVY DSISGVALAL 

       130        140        150        160        170        180 
DLTARNVQDE AKKKGLPWTI SKGFDTFMPI SAIVSREKFS SYKSNLQDIF RVKCSVNGQL 

       190        200        210        220        230        240 
RQDGGTNLML HPLHKILQHI STMISLEPGD IILTGTPAGV GELKPGDRVH CELLQNNDNI 

       250 
VDMNFECENR PGPYEFRET 

References and documentation are available.

Number of amino acids: 259

Molecular weight: 28792.07

Theoretical pI: 8.80

Amino acid composition: 
Ala (A)  12	  4.6%
Arg (R)  11	  4.2%
Asn (N)  18	  6.9%
Asp (D)  11	  4.2%
Cys (C)   4	  1.5%
Gln (Q)   7	  2.7%
Glu (E)  15	  5.8%
Gly (G)  17	  6.6%
His (H)   8	  3.1%
Ile (I)  20	  7.7%
Leu (L)  24	  9.3%
Lys (K)  19	  7.3%
Met (M)   6	  2.3%
Phe (F)  11	  4.2%
Pro (P)  17	  6.6%
Ser (S)  21	  8.1%
Thr (T)  16	  6.2%
Trp (W)   1	  0.4%
Tyr (Y)   6	  2.3%
Val (V)  15	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1281
Hydrogen    H	      2045
Nitrogen    N	       353
Oxygen      O	       380
Sulfur      S	        10

Formula: C1281H2045N353O380S10
Total number of atoms: 4069

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14690
Abs 0.1% (=1 g/l)   0.510, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.502, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.46
This classifies the protein as stable.



Aliphatic index: 87.68

Grand average of hydropathicity (GRAVY): -0.310