ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYPEKFEGI AIQSHEDWKN PKKTKYDPKP FYDHDIDIKI EACGVCGSDI HCAAGHWGNM 

        70         80         90        100        110        120 
KMPLVVGHEI VGKVVKLGPK SNSGLKVGQR VGVGAQVFSC LECDRCKNDN EPYCTKFVTT 

       130        140        150        160        170        180 
YSQPYEDGYV SQGGYANYVR VHEHFVVPIP ENIPSHLAAP LLCGGLTVYS PLVRNGCGPG 

       190        200        210        220        230        240 
KKVGIVGLGG IGSMGTLISK AMGAETYVIS RSSRKREDAM KMGADHYIAT LEEGDWGEKY 

       250        260        270        280        290        300 
FDTFDLIVVC ASSLTDIDFN IMPKAMKVGG RIVSISIPEQ HEMLSLKPYG LKAVSISYSA 

       310        320        330        340        350        360 
LGSIKELNQL LKLVSEKDIK IWVETLPVGE AGVHEAFERM EKGDVRYRFT LVGYDKEFSD 

References and documentation are available.

Number of amino acids: 360

Molecular weight: 39617.52

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  19	  5.3%
Arg (R)  11	  3.1%
Asn (N)  10	  2.8%
Asp (D)  20	  5.6%
Cys (C)  10	  2.8%
Gln (Q)   7	  1.9%
Glu (E)  25	  6.9%
Gly (G)  38	 10.6%
His (H)  11	  3.1%
Ile (I)  24	  6.7%
Leu (L)  24	  6.7%
Lys (K)  30	  8.3%
Met (M)  11	  3.1%
Phe (F)  11	  3.1%
Pro (P)  18	  5.0%
Ser (S)  26	  7.2%
Thr (T)  12	  3.3%
Trp (W)   4	  1.1%
Tyr (Y)  17	  4.7%
Val (V)  32	  8.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1770
Hydrogen    H	      2768
Nitrogen    N	       466
Oxygen      O	       523
Sulfur      S	        21

Formula: C1770H2768N466O523S21
Total number of atoms: 5548

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47955
Abs 0.1% (=1 g/l)   1.210, assuming all pairs of Cys residues form cystines


Ext. coefficient    47330
Abs 0.1% (=1 g/l)   1.195, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.75
This classifies the protein as stable.



Aliphatic index: 83.06

Grand average of hydropathicity (GRAVY): -0.203