ProtParam
User-provided sequence:
10 20 30 40 50 60
MSYRDNEYSG NYNGKEDGYN SRGRYGGGYR NNYSRGGGRG GFNDGASYGY DQRGQGRNFY
70 80 90 100 110 120
ESDGPGANLV KKDWKNETLI PFQKDFYKEH ENVRNRSDAE VTEYRKEKEI VVHGLNVPKP
130 140 150 160 170 180
VTTFEEAGFP NYVLKEVKQL GFEAPTPIQQ QAWPMAMSGR DMVGISATGS GKTLSYCLPA
190 200 210 220 230 240
IVHINAQPLL SPGDGPIVLV LAPTRELAVQ IQQECTKFGK SSRIRNTCVY GGVPLGPQIL
250 260 270 280 290 300
DLIRGVEICI ATPGRLLDML DSNKTNLRRV TYLVLDEADR MLDMGFEPQI RKIVDQIRPD
310 320 330 340 350 360
RQTVMFSATW PKEVQRLARD YLNDYIQVTV GSLDLAASHN IKQIVEVVDN ADKRARLGKD
370 380 390 400 410 420
IEEVLKDRDN KVLIFTGTKR VADDITRFLR QDGWPALAIH GDKAQDERDW VLNEFRTGKS
430 440 450 460 470 480
PIMVATDVAS RGIDVKGITH VFNYDFPGNT EDYVHRIGRT GRAGAKGTAY TYFTSDNAKQ
490 500 510 520 530 540
ARELVSILSE AKQDIDPKLE EMARYSSGGR GGNYRRGGYG RGGFRRGGGY GNRNRGFTGS
550
NSAPLARSRW
References and
documentation are available.
Number of amino acids: 550
Molecular weight: 61462.01
Theoretical pI: 9.13
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 79
Atomic composition:
Carbon C 2696
Hydrogen H 4239
Nitrogen N 799
Oxygen O 823
Sulfur S 14
Formula: C
2696H
4239N
799O
823S
14
Total number of atoms: 8571
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71990
Abs 0.1% (=1 g/l) 1.171, assuming all pairs of Cys residues form cystines
Ext. coefficient 71740
Abs 0.1% (=1 g/l) 1.167, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.68
This classifies the protein as stable.
Aliphatic index: 71.96
Grand average of hydropathicity (GRAVY): -0.659