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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYRDNEYSG NYNGKEDGYN SRGRYGGGYR NNYSRGGGRG GFNDGASYGY DQRGQGRNFY

70 80 90 100 110 120
ESDGPGANLV KKDWKNETLI PFQKDFYKEH ENVRNRSDAE VTEYRKEKEI VVHGLNVPKP

130 140 150 160 170 180
VTTFEEAGFP NYVLKEVKQL GFEAPTPIQQ QAWPMAMSGR DMVGISATGS GKTLSYCLPA

190 200 210 220 230 240
IVHINAQPLL SPGDGPIVLV LAPTRELAVQ IQQECTKFGK SSRIRNTCVY GGVPRGPQIR

250 260 270 280 290 300
DLIRGVEICI ATPGRLLDML DSNKTNLRRV TYLVLDEADR MLDMGFEPQI RKIVDQIRPD

310 320 330 340 350 360
RQTVMFSATW PKEVQRLARD YLNDYIQVTV GSLDLAASHN IKQIVEVVDN ADKRARLGKD

370 380 390 400 410 420
IEEVLKDRDN KVLIFTGTKR VADDITRFLR QDGWPALAIH GDKAQDERDW VLNEFRTGKS

430 440 450 460 470 480
PIMVATDVAS RGIDVKGITH VFNYDFPGNT EDYVHRIGRT GRAGAKGTAY TYFTSDNAKQ

490 500 510 520 530 540
ARELVSILSE AKQDIDPKLE EMARYSSGGR GGNYRRGGYG RGGFRRGGGY GNRNRGFTGS

550
NSAPLARSRW


References and documentation are available.

Number of amino acids: 550 Molecular weight: 61548.07 Theoretical pI: 9.24
Amino acid composition: 
Ala (A) 35 6.4% Arg (R) 51 9.3% Asn (N) 30 5.5% Asp (D) 39 7.1% Cys (C) 4 0.7% Gln (Q) 22 4.0% Glu (E) 30 5.5% Gly (G) 62 11.3% His (H) 7 1.3% Ile (I) 29 5.3% Leu (L) 34 6.2% Lys (K) 30 5.5% Met (M) 10 1.8% Phe (F) 18 3.3% Pro (P) 24 4.4% Ser (S) 28 5.1% Thr (T) 28 5.1% Trp (W) 6 1.1% Tyr (Y) 26 4.7% Val (V) 37 6.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 69 Total number of positively charged residues (Arg + Lys): 81 Atomic composition: Carbon C 2696 Hydrogen H 4241 Nitrogen N 805 Oxygen O 823 Sulfur S 14 Formula: C2696H4241N805O823S14 Total number of atoms: 8579 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 71990 Abs 0.1% (=1 g/l) 1.170, assuming all pairs of Cys residues form cystines Ext. coefficient 71740 Abs 0.1% (=1 g/l) 1.166, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 35.03 This classifies the protein as stable. Aliphatic index: 70.55 Grand average of hydropathicity (GRAVY): -0.690