ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSYSAADNLQ DSFQRAMNFS GSPGAVSTSP TQSFMNTLPR RVSITKQPKA LKPFSTGDMN 

        70         80         90        100        110        120 
ILLLENVNAT AIKIFKDQGY QVEFHKSSLP EDELIEKIKD VHAIGIRSKT RLTEKILQHA 

       130        140        150        160        170        180 
RNLVCIGCFC IGTNQVDLKY AASKGIAVFN SPFSNSRSVA ELVIGEIISL ARQLGDRSIE 

       190        200        210        220        230        240 
LHTGTWNKVA ARCWEVRGKT LGIIGYGHIG SQLSVLAEAM GLHVLYYDIV TIMALGTARQ 

       250        260        270        280        290        300 
VSTLDELLNK SDFVTLHVPA TPETEKMLSA PQFAAMKDGA YVINASRGTV VDIPSLIQAV 

       310        320        330        340        350        360 
KANKIAGAAL DVYPHEPAKN GEGSFNDELN SWTSELVSLP NIILTPHIGG STEEAQSSIG 

       370        380        390        400        410        420 
IEVATALSKY INEGNSVGSV NFPEVSLKSL DYDQENTVRV LYIHRNVPGV LKTVNDILSD 

       430        440        450        460 
HNIEKQFSDS HGEIAYLMAD ISSVNQSEIK DIYEKLNQTS AKVSIRLLY 

References and documentation are available.

Number of amino acids: 469

Molecular weight: 51188.27

Theoretical pI: 5.98

Amino acid composition: 
Ala (A)  38	  8.1%
Arg (R)  16	  3.4%
Asn (N)  27	  5.8%
Asp (D)  22	  4.7%
Cys (C)   4	  0.9%
Gln (Q)  18	  3.8%
Glu (E)  28	  6.0%
Gly (G)  30	  6.4%
His (H)  12	  2.6%
Ile (I)  39	  8.3%
Leu (L)  43	  9.2%
Lys (K)  27	  5.8%
Met (M)   9	  1.9%
Phe (F)  14	  3.0%
Pro (P)  17	  3.6%
Ser (S)  47	 10.0%
Thr (T)  26	  5.5%
Trp (W)   3	  0.6%
Tyr (Y)  14	  3.0%
Val (V)  35	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      2269
Hydrogen    H	      3630
Nitrogen    N	       616
Oxygen      O	       702
Sulfur      S	        13

Formula: C2269H3630N616O702S13
Total number of atoms: 7230

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37610
Abs 0.1% (=1 g/l)   0.735, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.730, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.17
This classifies the protein as unstable.



Aliphatic index: 97.93

Grand average of hydropathicity (GRAVY): -0.093