ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA RALDDFGVDY 

        70         80         90        100        110        120 
IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK VAVETGVDGV DVVIGTSKFL 

       130        140        150        160        170        180 
RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV 

       190        200        210        220        230        240 
GIADTVGCAN PRQVYELIRT LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL 

       250        260        270        280        290        300 
GIGERNGITP LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC 

       310        320        330        340        350        360 
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV DQLNLNLTDD 

       370        380        390        400        410        420 
QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL SAKKNKKNDS DVPELATIPA 


AKRTKPSA 

References and documentation are available.

Number of amino acids: 428

Molecular weight: 47098.90

Theoretical pI: 6.84

Amino acid composition: 
Ala (A)  42	  9.8%
Arg (R)  19	  4.4%
Asn (N)  25	  5.8%
Asp (D)  34	  7.9%
Cys (C)   8	  1.9%
Gln (Q)   8	  1.9%
Glu (E)  22	  5.1%
Gly (G)  25	  5.8%
His (H)  11	  2.6%
Ile (I)  37	  8.6%
Leu (L)  31	  7.2%
Lys (K)  36	  8.4%
Met (M)   6	  1.4%
Phe (F)  16	  3.7%
Pro (P)  15	  3.5%
Ser (S)  24	  5.6%
Thr (T)  22	  5.1%
Trp (W)   1	  0.2%
Tyr (Y)  12	  2.8%
Val (V)  34	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2088
Hydrogen    H	      3351
Nitrogen    N	       577
Oxygen      O	       632
Sulfur      S	        14

Formula: C2088H3351N577O632S14
Total number of atoms: 6662

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23880
Abs 0.1% (=1 g/l)   0.507, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.496, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.87
This classifies the protein as stable.



Aliphatic index: 94.81

Grand average of hydropathicity (GRAVY): -0.184