ProtParam
User-provided sequence:
10 20 30 40 50 60
MTAATTSQPA FSPDQVSVIF VLGGPGAGKG TQCEKLVKDY SFVHLSAGDL LRAEQGRAGS
70 80 90 100 110 120
QYGELIKNCI KEGQIVPQEI TLALLRNAIS DNVKANKHKF LIDGFPRKMD QAISFERDIV
130 140 150 160 170 180
ESKFILFFDC PEDIMLERLL ERGKTSGRSD DNIESIKKRF NTFKETSMPV IEYFETKSKV
190 200
VRVRCDRSVE DVYKDVQDAI RDSL
References and
documentation are available.
Number of amino acids: 204
Molecular weight: 22933.20
Theoretical pI: 6.19
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1012
Hydrogen H 1625
Nitrogen N 279
Oxygen O 311
Sulfur S 8
Formula: C
1012H
1625N
279O
311S
8
Total number of atoms: 3235
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6210
Abs 0.1% (=1 g/l) 0.271, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.260, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.72
This classifies the protein as unstable.
Aliphatic index: 84.56
Grand average of hydropathicity (GRAVY): -0.356