ProtParam
User-provided sequence:
10 20 30 40 50 60
MTAEDILLRE RTSTTTQRPV NSEQYLNVQL ATAPVKNFQT TSEISRQTLV DTSNDDVYSI
70 80 90 100 110 120
KNLKGSRNPI SPSVSNVGFQ SIFHTVDHPR SKVSVASNHS LRSNDNASAA TSKSGSSQIG
130 140 150 160 170 180
ESHSVDTVEC SNNLSKKLSS DAISITQKSL HSTPSGRYMK GKASGFFNRR NRAHTTISSD
190 200 210 220 230 240
PASFLTDSST LHNSSHSFRN VIKNFFQNKS HRHIGQDAIE PAIPNSLSKF LHSSYGRHKS
250 260 270 280 290 300
PSQFIHTNAG QLVDSGTSVY SLNVNPSGVN PNTIVEDPLS GTDPASPNPV SMLHDLLRNL
310 320 330 340 350 360
PSLEANYKHF NSQELTTLTN NIWNIFCSNV AELFRTQRIW KLRAKIENFN EVLEFYCILK
370 380 390 400 410 420
TDPRVTHSGM NRIISDLKEF LVSSLYNLEN QIVFNYSNED TINNALKRLG VIWRIFYQEV
430 440 450 460 470 480
YYDLAAVLLP LDQSIREDGN STVLKSGNES RTHINGNYSI GFLLLMCFRD SIVLPCYENF
490 500 510 520 530 540
VNSNDGISKS FQLYIFNQEE ESNVTETDKL TLLQCFGILS TIQSNDRNQR IIEELLAGIR
MSI
References and
documentation are available.
Number of amino acids: 543
Molecular weight: 60840.95
Theoretical pI: 7.00
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2664
Hydrogen H 4199
Nitrogen N 761
Oxygen O 848
Sulfur S 12
Formula: C
2664H
4199N
761O
848S
12
Total number of atoms: 8484
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39225
Abs 0.1% (=1 g/l) 0.645, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.639, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.59
This classifies the protein as unstable.
Aliphatic index: 83.81
Grand average of hydropathicity (GRAVY): -0.427