ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTAEEFDFSS HSHRRYNPLT DSWILVSPHR AKRPWLGQQE AAYKPTAPLY DPKCYLCPGN 

        70         80         90        100        110        120 
KRATGNLNPR YESTYIFPND YAAVRLDQPI LPQNDSNEDN LKNRLLKVQS VRGNCFVICF 

       130        140        150        160        170        180 
SPNHNLTIPQ MKQSDLVHIV NSWQALTDDL SREARENHKP FKYVQIFENK GTAMGCSNLH 

       190        200        210        220        230        240 
PHGQAWCLES IPSEVSQELK SFDKYKREHN TDLFADYVKL ESREKSRVVV ENESFIVVVP 

       250        260        270        280        290        300 
YWAIWPFETL VISKKKLASI SQFNQMVKED LASILKQLTI KYDNLFETSF PYSMGIHQAP 

       310        320        330        340        350        360 
LNATGDELSN SWFHMHFYPP LLRSATVRKF LVGFELLGEP QRDLTSEQAA EKLRNLDGQI 


HYLQRL 

References and documentation are available.

Number of amino acids: 366

Molecular weight: 42385.10

Theoretical pI: 7.21

Amino acid composition: 
Ala (A)  21	  5.7%
Arg (R)  19	  5.2%
Asn (N)  23	  6.3%
Asp (D)  18	  4.9%
Cys (C)   6	  1.6%
Gln (Q)  19	  5.2%
Glu (E)  24	  6.6%
Gly (G)  12	  3.3%
His (H)  13	  3.6%
Ile (I)  16	  4.4%
Leu (L)  38	 10.4%
Lys (K)  23	  6.3%
Met (M)   6	  1.6%
Phe (F)  18	  4.9%
Pro (P)  23	  6.3%
Ser (S)  30	  8.2%
Thr (T)  15	  4.1%
Trp (W)   7	  1.9%
Tyr (Y)  15	  4.1%
Val (V)  20	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1907
Hydrogen    H	      2927
Nitrogen    N	       521
Oxygen      O	       553
Sulfur      S	        12

Formula: C1907H2927N521O553S12
Total number of atoms: 5920

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61225
Abs 0.1% (=1 g/l)   1.444, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   1.436, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.02
This classifies the protein as unstable.



Aliphatic index: 79.13

Grand average of hydropathicity (GRAVY): -0.540