ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTAPHRSTIH ILGLGAMGTV LAVDLLRFTN ALVVPLFRSQ ERLAQFQKTN GNNISIRKLY 

        70         80         90        100        110        120 
LEGSPIFSYP VEKCECPETF SKKPIDNLVV TTKTYQTKEA LAPYLPYINK NTNLILIQNG 

       130        140        150        160        170        180 
LGVLELLREE IFTDSKNRPH LFQGVISHGV YQDKAGVFNH AGWAGMKIAK LPWTEEEMIQ 

       190        200        210        220        230        240 
KKSVVEDDAA NNSLVKLLTE PKFAKEFGIE HSTYQEMLFG QLFKFLVNAC MNPVTAILDC 

       250        260        270        280        290        300 
VNGEMKASCG PVFTSIIDEC LQILRVAYRP LFQYHEKYSG NEEYPEMDVN AVLTTDNMVS 

       310        320        330        340        350        360 
EVTRIGCDIN SRNSSSMRQD TLFLRDIEIE YINGYVVKLA DNLNLDPNCC KVNKTIGELA 

       370 
TMRLALNRSR SINGDWRKD 

References and documentation are available.

Number of amino acids: 379

Molecular weight: 42821.28

Theoretical pI: 6.21

Amino acid composition: 
Ala (A)  22	  5.8%
Arg (R)  17	  4.5%
Asn (N)  28	  7.4%
Asp (D)  17	  4.5%
Cys (C)   9	  2.4%
Gln (Q)  13	  3.4%
Glu (E)  27	  7.1%
Gly (G)  21	  5.5%
His (H)   7	  1.8%
Ile (I)  25	  6.6%
Leu (L)  40	 10.6%
Lys (K)  24	  6.3%
Met (M)  11	  2.9%
Phe (F)  16	  4.2%
Pro (P)  16	  4.2%
Ser (S)  21	  5.5%
Thr (T)  23	  6.1%
Trp (W)   3	  0.8%
Tyr (Y)  13	  3.4%
Val (V)  26	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1907
Hydrogen    H	      3024
Nitrogen    N	       512
Oxygen      O	       566
Sulfur      S	        20

Formula: C1907H3024N512O566S20
Total number of atoms: 6029

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36370
Abs 0.1% (=1 g/l)   0.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.838, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.98
This classifies the protein as unstable.



Aliphatic index: 92.59

Grand average of hydropathicity (GRAVY): -0.198