ProtParam
User-provided sequence:
10 20 30 40 50 60
MTAPLVVLGN PLLDFQADVT AEYLAKYSLK ENDAILVDAK SGDAKMAIFD ELLQMPETKL
70 80 90 100 110 120
VAGGAAQNTA RGAAYVLGAG QVVYFGSVGK DKFSERLLNE NEKAGVKSMY QVQNDIGTGK
130 140 150 160 170 180
CAALITGHNR SLVTDLGAAN FFTPDHLDKH WDLVEAAKLF YIGGFHLTVS PDAIVKLGQH
190 200 210 220 230 240
AKENSKPFVL NFSAPFIPHV FKDALARVLP YATVIIANES EAEAFCDAFQ LDCANTDLEA
250 260 270 280 290 300
IAQRIVKDSP VEKTVIFTHG VEPTVVVSSK GTSTYPVKPL DSSKIVDTNG AGDAFAGGFM
310 320 330 340
AGLTKGEDLE TSIDMGQWLA ALSIQEVGPS YPSEKISYSK
References and
documentation are available.
Number of amino acids: 340
Molecular weight: 36372.34
Theoretical pI: 4.99
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1635
Hydrogen H 2557
Nitrogen N 419
Oxygen O 500
Sulfur S 9
Formula: C
1635H
2557N
419O
500S
9
Total number of atoms: 5120
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26025
Abs 0.1% (=1 g/l) 0.716, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.712, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.59
This classifies the protein as stable.
Aliphatic index: 90.71
Grand average of hydropathicity (GRAVY): 0.006