ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTAQQGVPIK ITNKEIAQEF LDKYDTFLFD CDGVLWLGSQ ALPYTLEILN LLKQLGKQLI 

        70         80         90        100        110        120 
FVTNNSTKSR LAYTKKFASF GIDVKEEQIF TSGYASAVYI RDFLKLQPGK DKVWVFGESG 

       130        140        150        160        170        180 
IGEELKLMGY ESLGGADSRL DTPFDAAKSP FLVNGLDKDV SCVIAGLDTK VNYHRLAVTL 

       190        200        210        220        230        240 
QYLQKDSVHF VGTNVDSTFP QKGYTFPGAG SMIESLAFSS NRRPSYCGKP NQNMLNSIIS 

       250        260        270        280        290        300 
AFNLDRSKCC MVGDRLNTDM KFGVEGGLGG TLLVLSGIET EERALKISHD YPRPKFYIDK 

       310 
LGDIYTLTNN EL 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 34624.58

Theoretical pI: 6.00

Amino acid composition: 
Ala (A)  16	  5.1%
Arg (R)  10	  3.2%
Asn (N)  15	  4.8%
Asp (D)  21	  6.7%
Cys (C)   5	  1.6%
Gln (Q)  12	  3.8%
Glu (E)  15	  4.8%
Gly (G)  28	  9.0%
His (H)   3	  1.0%
Ile (I)  17	  5.4%
Leu (L)  36	 11.5%
Lys (K)  24	  7.7%
Met (M)   6	  1.9%
Phe (F)  18	  5.8%
Pro (P)  11	  3.5%
Ser (S)  23	  7.4%
Thr (T)  19	  6.1%
Trp (W)   2	  0.6%
Tyr (Y)  13	  4.2%
Val (V)  18	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1559
Hydrogen    H	      2439
Nitrogen    N	       401
Oxygen      O	       467
Sulfur      S	        11

Formula: C1559H2439N401O467S11
Total number of atoms: 4877

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30620
Abs 0.1% (=1 g/l)   0.884, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.877, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.74
This classifies the protein as stable.



Aliphatic index: 88.11

Grand average of hydropathicity (GRAVY): -0.179