ProtParam
User-provided sequence:
10 20 30 40 50 60
MTASLTTKFL NNTYENPFMN ASGVHCMTTQ ELDELANSKA GAFITKSATT LEREGNPEPR
70 80 90 100 110 120
YISVPLGSIN SMGLPNEGID YYLSYVLNRQ KNYPDAPAIF FSVAGMSIDE NLNLLRKIQD
130 140 150 160 170 180
SEFNGITELN LSCPNVPGKP QVAYDFDLTK ETLEKVFAFF KKPLGVKLPP YFDFAHFDIM
190 200 210 220 230 240
AKILNEFPLA YVNSINSIGN GLFIDVEKES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT
250 260 270 280 290 300
RLRPEIKVIG TGGIKSGKDA FEHLLCGASM LQIGTELQKE GVKIFERIEK ELKDIMEAKG
310
YTSIDQFRGK LNSI
References and
documentation are available.
Number of amino acids: 314
Molecular weight: 34800.93
Theoretical pI: 5.80
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1573
Hydrogen H 2463
Nitrogen N 401
Oxygen O 466
Sulfur S 11
Formula: C
1573H
2463N
401O
466S
11
Total number of atoms: 4914
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18005
Abs 0.1% (=1 g/l) 0.517, assuming all pairs of Cys residues form cystines
Ext. coefficient 17880
Abs 0.1% (=1 g/l) 0.514, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.73
This classifies the protein as stable.
Aliphatic index: 86.66
Grand average of hydropathicity (GRAVY): -0.188