ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTASLTTKFL NNTYENPFMN ASGVHCMTTQ ELDELANSKA GAFITKSATT LEREGNPEPR 

        70         80         90        100        110        120 
YISVPLGSIN SMGLPNEGID YYLSYVLNRQ KNYPDAPAIF FSVAGMSIDE NLNLLRKIQD 

       130        140        150        160        170        180 
SEFNGITELN LSCPNVPGKP QVAYDFDLTK ETLEKVFAFF KKPLGVKLPP YFDFAHFDIM 

       190        200        210        220        230        240 
AKILNEFPLA YVNSINSIGN GLFIDVEKES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT 

       250        260        270        280        290        300 
RLRPEIKVIG TGGIKSGKDA FEHLLCGASM LQIGTELQKE GVKIFERIEK ELKDIMEAKG 

       310 
YTSIDQFRGK LNSI 

References and documentation are available.

Number of amino acids: 314

Molecular weight: 34800.93

Theoretical pI: 5.80

Amino acid composition: 
Ala (A)  20	  6.4%
Arg (R)   9	  2.9%
Asn (N)  22	  7.0%
Asp (D)  13	  4.1%
Cys (C)   3	  1.0%
Gln (Q)   7	  2.2%
Glu (E)  24	  7.6%
Gly (G)  26	  8.3%
His (H)   3	  1.0%
Ile (I)  23	  7.3%
Leu (L)  29	  9.2%
Lys (K)  25	  8.0%
Met (M)   8	  2.5%
Phe (F)  20	  6.4%
Pro (P)  17	  5.4%
Ser (S)  19	  6.1%
Thr (T)  17	  5.4%
Trp (W)   0	  0.0%
Tyr (Y)  12	  3.8%
Val (V)  17	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1573
Hydrogen    H	      2463
Nitrogen    N	       401
Oxygen      O	       466
Sulfur      S	        11

Formula: C1573H2463N401O466S11
Total number of atoms: 4914

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18005
Abs 0.1% (=1 g/l)   0.517, assuming all pairs of Cys residues form cystines


Ext. coefficient    17880
Abs 0.1% (=1 g/l)   0.514, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.73
This classifies the protein as stable.



Aliphatic index: 86.66

Grand average of hydropathicity (GRAVY): -0.188