ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA EMQQIRNTVY 

        70         80         90        100        110        120 
ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR QQQQQQQVQQ HLQQQQQQLA 

       130        140        150        160        170        180 
AASASVPVAQ QPPATTSATA TPAANTTTGS PSAFPVQASR PNLVGSQLPT TTLPVVSSNA 

       190        200        210        220        230        240 
QQQLPQQQLQ QQQLQQQQPP PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP 

       250        260        270        280        290        300 
ENNNTSKIND TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP 

       310        320        330        340        350        360 
FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV KFSNDGEYLA 

       370        380        390        400        410        420 
TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT TSTDNNTMTT TTTTTITTTA 

       430        440        450        460        470        480 
MTSAAELAKD VENLNTSSSP SSDLYIRSVC FSPDGKFLAT GAEDRLIRIW DIENRKIVMI 

       490        500        510        520        530        540 
LQGHEQDIYS LDYFPSGDKL VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK 

       550        560        570        580        590        600 
YIAAGSLDRA VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV 

       610        620        630        640        650        660 
KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK DRGVLFWDKK 

       670        680        690        700        710 
SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC KARIWKYKKI APN 

References and documentation are available.

Number of amino acids: 713

Molecular weight: 78307.69

Theoretical pI: 5.43

Amino acid composition: 
Ala (A)  51	  7.2%
Arg (R)  27	  3.8%
Asn (N)  44	  6.2%
Asp (D)  43	  6.0%
Cys (C)   7	  1.0%
Gln (Q)  61	  8.6%
Glu (E)  36	  5.0%
Gly (G)  34	  4.8%
His (H)  14	  2.0%
Ile (I)  27	  3.8%
Leu (L)  60	  8.4%
Lys (K)  36	  5.0%
Met (M)   8	  1.1%
Phe (F)  13	  1.8%
Pro (P)  36	  5.0%
Ser (S)  70	  9.8%
Thr (T)  72	 10.1%
Trp (W)   6	  0.8%
Tyr (Y)  20	  2.8%
Val (V)  48	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      3380
Hydrogen    H	      5391
Nitrogen    N	       969
Oxygen      O	      1139
Sulfur      S	        15

Formula: C3380H5391N969O1139S15
Total number of atoms: 10894

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63175
Abs 0.1% (=1 g/l)   0.807, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   0.802, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.80
This classifies the protein as unstable.



Aliphatic index: 74.26

Grand average of hydropathicity (GRAVY): -0.628