ProtParam
User-provided sequence:
10 20 30 40 50 60
MTAVSANGKT TEKHENGAHT NGTTNGTTNG SMNGNEISHV QKLQPVYYKP PQNLETFELS
70 80 90 100 110 120
LRNHFEEKTN KKFADYREFH RFTCDNYGIF WEDLLKLSDV KLHQNYNQVI DHNLKINERP
130 140 150 160 170 180
RWFNGATLNY TENVIERGTA TDIAVLNASI EETVTEYTYD NLRKDVYRIA TSLRNYGIGP
190 200 210 220 230 240
GDTVCGFVPN TYDTLVAVFA TAAVGAAWCS ASVDFGPAGV LDRFRQVHPK VLFTVNHVTY
250 260 270 280 290 300
KKKLIDQTDK INEIVKELPT LEKIVVSDTF TSVKFDATKY NQSDKFSSLE EFKTPIADVV
310 320 330 340 350 360
LPFVYTPVPF SDPLFVMFSS GTTGIPKAMV HTVGGTLLKH IEEHLVQGDS KKHDRMFFYT
370 380 390 400 410 420
TCGWMMYNWM ISFLYSKGSV VLFDECPLAP DTHIIMKIAA KTQSTMIGMG AKLYDEYLRL
430 440 450 460 470 480
QIPFNTLYDL SKIHTVYSTG SPLKKECFAY INTYIAPGAL IASISGGTDI IGCFVGGIKS
490 500 510 520 530 540
LSITPGECQC LFLGMDIKSF NYMDEEIINS DEQGELVCVT PFPSMPSHFL NDTDGKKYRD
550 560 570 580 590 600
AYFARLEPFW AHGDFVRVNH STGGVEMLGR SDATLNRGGV RIGTAEIYSV VEKIPHIADC
610 620 630 640 650 660
IVAGRLVEEG MDEEVLLFVK MVPGQELTHS IQAAIVSKLR NDMSPRHVPN KIYAVDDIPY
670 680 690 700
TSSGKKVEVA VKQIVSGKAV QKASSIRNPE SLDHFVQYRL
References and
documentation are available.
Number of amino acids: 700
Molecular weight: 78339.28
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 70
Atomic composition:
Carbon C 3519
Hydrogen H 5450
Nitrogen N 922
Oxygen O 1046
Sulfur S 29
Formula: C
3519H
5450N
922O
1046S
29
Total number of atoms: 10966
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78325
Abs 0.1% (=1 g/l) 1.000, assuming all pairs of Cys residues form cystines
Ext. coefficient 77700
Abs 0.1% (=1 g/l) 0.992, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.82
This classifies the protein as stable.
Aliphatic index: 82.81
Grand average of hydropathicity (GRAVY): -0.207