ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTAYSGSPSK PGNNNSYLNR YVENLGTNVT PPLRPQSSSK INSSLNIASP SHLKTKTSAS 

        70         80         90        100        110        120 
NSSATILSKK VESSVSKLKP SLPNKLVGKY TVDLSNYSKI ELRYYEFLCR VSEVKIWIEA 

       130        140        150        160        170        180 
VIEEALPSEI ELCVGDSLRN GVFLAKLTQR INPDLTTVIF PAGDKLQFKH TQNINAFFGL 

       190        200        210        220        230        240 
VEHVGVPDSF RFELQDLYNK KNIPQVFETL HILISMINKK WPGKTPALTN VSGQISFTKE 

       250        260        270        280        290        300 
EIAACKKAWP RIRDFKSLGT NINTAPASPE EPKEKRSGLI KDFNKFERPN IPVEEILITP 

       310        320        330        340        350        360 
RKNITDANCS DFSNTPSPYN EAPKMSNLDV VVEKRKFTPI EPSLLGPTPS LEYSPIKNKS 

       370        380        390        400        410        420 
LSYYSPTISK YLTYDTEFYT RRSRAREEDL NYYQTFKYSP SHYSPMRRER MTEEQFLEKV 

       430        440        450        460        470        480 
VQLQNICRGV NTRFNLYIQK RLLNLFEQDI LRFQACLRGN KFRVLSSMYL PIRRAKIDVP 

       490        500        510        520        530        540 
HVEAIQSRIK GSRIRYKYDK LKFTLSRFSC TVELLQAYCR SKLLKTTVNT KLNDIEISHY 

       550        560        570        580        590        600 
PLTKLQSYMR ASYVRKKVMS LNTKLNDERE SIMKFSAIIR GNVVRCSEDA ILSAVHDVHK 

       610        620        630        640        650        660 
ENISKLQSLI RGIFTRSCLA SIIYSLGKEN CNIIQLSACI RGNAVRHKVQ SLFAPENNLS 

       670        680        690        700        710        720 
ETVHDLQGLV RGILVRYTLD LVDDIVEYNN LALFQAFSRG ALVRESLDQK SSFYKRNVRS 

       730        740        750        760        770        780 
VIMIQSWIRK SLQRSAYLEL LDCPNPSLWA VKKFVHLLNG TATIEEVQNQ LESCQASLDS 

       790        800        810        820        830        840 
ENMKKERLLK SIRQQLNING VLDKFGLLKD KDHELGISDS TIPKSKYQKY EKLFYMLQVD 

       850        860        870        880        890        900 
PSYWKLLYLK EPEFVAKNVY MTFGTVNQRM NDRERSYFTR FVCEMLQNAI NEAPSIESFL 

       910        920        930        940        950        960 
DNRSQFWQTI LQDFLRRESP EFFSIIVPVL DYLSDPVVDF ESDPYKIYQE IHGFSSPQHC 

       970        980        990       1000       1010       1020 
SPVDDASTKN KFIDNLRCLW HAIEMVAEIY TRKVHTIPVE IRYLCTKIFC YAADKNIEEI 

      1030       1040       1050       1060       1070       1080 
DSLRAISSIL VNVFVSEYLV NREYYGYKDS NVQKNNQKID ILMKSLATVF EIKNFDGFLD 

      1090       1100       1110       1120       1130       1140 
PLNQYANEIK PHIKDVLYNV LVDPEYEQEG DRLIYLDMVS PSPKLELLTE KVLEISGKFE 

      1150       1160       1170       1180       1190       1200 
EYLNEFPEAD ILHDILEKNL DNSSFPRSGR VTLELDASAY RFLVSDDKMR KIYDQVKRAF 

      1210       1220       1230       1240       1250       1260 
VYMMQIEDVD TNLYDLSIST ILPQDEPNFA NFLEQNPKIR DDPMIQKLKP LKYFTLKNVT 

      1270       1280       1290       1300       1310       1320 
LKKIHELEST GTFCSSDNKL QNFLNDIANT IKNPNYAIDY VTQEIYITKE TLTKISEMNH 

      1330       1340       1350       1360       1370       1380 
SLDIELSRLK KHVDHTIKDF QKAKDFSPVH KSKFGNFKNA VKKVQGRERS ELQGMKFKWN 

      1390       1400       1410       1420       1430       1440 
TKQLYERGVL KTIRGEKLAE LTVKVFGSSG PKFPDIIFKI STSDGSRFGI QMIDKRKGPD 

      1450       1460       1470       1480       1490 
KRYSDDVDSF SFKDLIKTQV EPKIETWKLF HSNVVVNNSQ LLHLIVSFFY KRNAL 

References and documentation are available.

Number of amino acids: 1495

Molecular weight: 172830.44

Theoretical pI: 9.04

Amino acid composition: 
Ala (A)  59	  3.9%
Arg (R)  80	  5.4%
Asn (N)  95	  6.4%
Asp (D)  80	  5.4%
Cys (C)  20	  1.3%
Gln (Q)  57	  3.8%
Glu (E) 100	  6.7%
Gly (G)  46	  3.1%
His (H)  26	  1.7%
Ile (I) 109	  7.3%
Leu (L) 151	 10.1%
Lys (K) 131	  8.8%
Met (M)  25	  1.7%
Phe (F)  74	  4.9%
Pro (P)  68	  4.5%
Ser (S) 135	  9.0%
Thr (T)  71	  4.7%
Trp (W)  10	  0.7%
Tyr (Y)  65	  4.3%
Val (V)  93	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 180
Total number of positively charged residues (Arg + Lys): 211

Atomic composition:

Carbon      C	      7776
Hydrogen    H	     12296
Nitrogen    N	      2080
Oxygen      O	      2279
Sulfur      S	        45

Formula: C7776H12296N2080O2279S45
Total number of atoms: 24476

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   153100
Abs 0.1% (=1 g/l)   0.886, assuming all pairs of Cys residues form cystines


Ext. coefficient   151850
Abs 0.1% (=1 g/l)   0.879, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.85
This classifies the protein as unstable.



Aliphatic index: 89.81

Grand average of hydropathicity (GRAVY): -0.421