ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDDEKREIL KEFDPFSQLE QANGNPDKDV KFKKDDPNRA AAEETNRDIS AQDKGDEEPF 

        70         80         90        100        110        120 
YDFQIFIKQL QTPGADPLVK YTKSFLRNFL AQRLLWTVSE EIKLISDFKT FIYDKFTLYE 

       130        140        150        160        170        180 
PFRSLDNSKM RNAKEGMEKL IMGKLYSRCF SPSLYEILQK PLDDEHMKDL TNDDTLLEKI 

       190        200        210        220        230        240 
RHYRFISPIM LDIPDTMPNA RLNKFVHLAS KELGKINRFK SPRDKMVCVL NASKVIFGLL 

       250        260        270        280        290        300 
KHTKLEQNGA DSFIPVLIYC ILKGQVRYLV SNVNYIERFR SPDFIRGEEE YYLSSLQAAL 

       310        320        330        340        350        360 
NFIMNLTERS LTIEDHEDFE EAYQRNFKQL AEEKEEEEKK KQLEIPDELQ PNGTLLKPLD 

       370        380        390        400        410        420 
EVTNIVISKF NELFSPIGEP TQEEALKSEQ SNKEEDVSSL IKKIEENERK DTLNTLQNMF 

       430        440        450 
PDMDPSLIED VCIAKKSRIG PCVDALLSLS E 

References and documentation are available.

Number of amino acids: 451

Molecular weight: 52482.77

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  19	  4.2%
Arg (R)  21	  4.7%
Asn (N)  25	  5.5%
Asp (D)  35	  7.8%
Cys (C)   5	  1.1%
Gln (Q)  18	  4.0%
Glu (E)  48	 10.6%
Gly (G)  13	  2.9%
His (H)   5	  1.1%
Ile (I)  31	  6.9%
Leu (L)  50	 11.1%
Lys (K)  43	  9.5%
Met (M)  11	  2.4%
Phe (F)  26	  5.8%
Pro (P)  23	  5.1%
Ser (S)  30	  6.7%
Thr (T)  18	  4.0%
Trp (W)   1	  0.2%
Tyr (Y)  13	  2.9%
Val (V)  16	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2342
Hydrogen    H	      3701
Nitrogen    N	       611
Oxygen      O	       722
Sulfur      S	        16

Formula: C2342H3701N611O722S16
Total number of atoms: 7392

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25120
Abs 0.1% (=1 g/l)   0.479, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.474, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.38
This classifies the protein as unstable.



Aliphatic index: 84.55

Grand average of hydropathicity (GRAVY): -0.618