ProtParam
User-provided sequence:
10 20 30 40 50 60
MTDFDLMNFP FHERLDSPVS ENGEIKDGEP IPQNWLNENH VGKSILPLFV NPEDVINCNF
70 80 90 100 110 120
SNARDSYEEN KSPSMDQMNY ARNTSYQESP GLQERPKNEK DKSPIGTDVH KKDVPNFIHS
130 140 150 160 170 180
TPRENSSKHF TRANEQASAQ PTDEHTSPDI SIEDCNGAKI FLQNSLSKED FRMLENVILG
190 200 210 220 230 240
YQKKVIELGR DNLRQEERAN SLQKELEAAT KSNDKTLDNK KKIEEQTVLI ENLTKDLSLN
250 260 270 280 290 300
KEMLEKANDT IQTKHTALLS LTDSLRKAEL FEIPIGILFF DLYDSEENSS KLDHILQEKY
310 320 330 340 350 360
PNIKGFLCAS QQEELSRISQ RFKNAKAEAE DLRNELENKK IEIQTMREKN NTLIGTNKTL
370 380 390 400 410 420
SKQNKILCDK FDKLTIDEKE ILKGCNEEIK IKLERLNERL GSWEKSKEKY ETSLKDKEKM
430 440 450 460 470 480
LADAEKKTNT LSKELDNLRS RFGNLEGNTS ERITIKNILQ SRPDISAEEC NFLMVEQIDS
490 500 510 520 530 540
ANLTTLQNTV KEIVLAVGIP YPKLRRKIPL LAIKLKYENI MLSNFAQRLH RQVYSQEMNL
550 560 570 580
KKFTDQAYYD FMSTRRMDSI DHHLERCLDH LYDHILEKMV K
References and
documentation are available.
Number of amino acids: 581
Molecular weight: 67400.22
Theoretical pI: 5.74
Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 88
Atomic composition:
Carbon C 2941
Hydrogen H 4740
Nitrogen N 826
Oxygen O 941
Sulfur S 21
Formula: C
2941H
4740N
826O
941S
21
Total number of atoms: 9469
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30745
Abs 0.1% (=1 g/l) 0.456, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.451, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.21
This classifies the protein as unstable.
Aliphatic index: 80.76
Grand average of hydropathicity (GRAVY): -0.850