ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDGKFPTNI EPKYIPYSKD HASLTACANP IPLDLKSLFP NNTVVVTAVP GAFTPTCTEQ 

        70         80         90        100        110        120 
HIPDYLKHLK DFKDKGVKKI IVLSANDPFV MAAWAKALGY TDEENYVIFA TDPNASISKE 

       130        140        150        160        170 
LGDGFVADLT SAGMGLRLQR YASIVVNGEI TYLETEDSLG FSEISSAETI LKRIHN 

References and documentation are available.

Number of amino acids: 176

Molecular weight: 19257.93

Theoretical pI: 5.16

Amino acid composition: 
Ala (A)  16	  9.1%
Arg (R)   3	  1.7%
Asn (N)   9	  5.1%
Asp (D)  12	  6.8%
Cys (C)   2	  1.1%
Gln (Q)   2	  1.1%
Glu (E)  10	  5.7%
Gly (G)  10	  5.7%
His (H)   4	  2.3%
Ile (I)  13	  7.4%
Leu (L)  15	  8.5%
Lys (K)  13	  7.4%
Met (M)   3	  1.7%
Phe (F)   8	  4.5%
Pro (P)  11	  6.2%
Ser (S)  12	  6.8%
Thr (T)  14	  8.0%
Trp (W)   1	  0.6%
Tyr (Y)   7	  4.0%
Val (V)  11	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       869
Hydrogen    H	      1356
Nitrogen    N	       218
Oxygen      O	       265
Sulfur      S	         5

Formula: C869H1356N218O265S5
Total number of atoms: 2713

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16055
Abs 0.1% (=1 g/l)   0.834, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.827, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.11
This classifies the protein as stable.



Aliphatic index: 89.26

Grand average of hydropathicity (GRAVY): -0.113