ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDIERFIEK LPKAELHLHL EGTLEAELKL KLSHRNKIPL KQSSIEEIKE SYNFHDLASF 

        70         80         90        100        110        120 
LEVYYEGVEL LLHEQDFYDL CYQYLRKAAS QNVVYAEMFF DPQLHTRRGI SFETVIKGLI 

       130        140        150        160        170        180 
RARDDAMRDF HIYSQLIMCF IREMSFENAE ETLNASLPYK SEIIGIGLDS NEENNPPIKF 

       190        200        210        220        230        240 
LKVFQRARQL GYRLTCHCDL HQKNTTTHIR QALEDIGVER IDHGINILDD PELIKLALER 

       250        260        270        280        290        300 
NIPFTVCPFS NEIVYPGKAQ PEIRIMLDTG LKVTINSDDP AYMHCFYITE NFNLAQKGAS 

       310        320        330 
LTKKELVQIC RNSFEAAWIS EEKRNHYLEA LNSFASAYD 

References and documentation are available.

Number of amino acids: 339

Molecular weight: 39436.00

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  22	  6.5%
Arg (R)  18	  5.3%
Asn (N)  18	  5.3%
Asp (D)  18	  5.3%
Cys (C)   7	  2.1%
Gln (Q)  13	  3.8%
Glu (E)  34	 10.0%
Gly (G)  12	  3.5%
His (H)  13	  3.8%
Ile (I)  29	  8.6%
Leu (L)  38	 11.2%
Lys (K)  20	  5.9%
Met (M)   7	  2.1%
Phe (F)  18	  5.3%
Pro (P)  12	  3.5%
Ser (S)  19	  5.6%
Thr (T)  14	  4.1%
Trp (W)   1	  0.3%
Tyr (Y)  15	  4.4%
Val (V)  11	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1769
Hydrogen    H	      2753
Nitrogen    N	       471
Oxygen      O	       523
Sulfur      S	        14

Formula: C1769H2753N471O523S14
Total number of atoms: 5530

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28225
Abs 0.1% (=1 g/l)   0.716, assuming all pairs of Cys residues form cystines


Ext. coefficient    27850
Abs 0.1% (=1 g/l)   0.706, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.85
This classifies the protein as unstable.



Aliphatic index: 92.98

Grand average of hydropathicity (GRAVY): -0.350