ProtParam
User-provided sequence:
10 20 30 40 50 60
MTDIQLNGKS TLDTPSATMS AKEKEAKLKS ADENNKPPNY KRISDDDLYR HSSQYRMWSY
70 80 90 100 110 120
TKDQLQEKRV DTNARAIAYI EENLLKFREA HNLTEEEIKV LEAKAIPLTM EEELDLVNFY
130 140 150 160 170 180
AKKVQVIAQH LNLPTEVVAT AISFFRRFFL ENSVMQIDPK SIVHTTIFLA CKSENYFISV
190 200 210 220 230 240
DSFAQKAKST RDSVLKFEFK LLESLKFSLL NHHPYKPLHG FFLDIQNVLY GKVDLNYMGQ
250 260 270 280 290 300
IYDRCKKRIT AALLTDVVYF YTPPQITLAT LLIEDEALVT RYLETKFPSR EGSQESVPGN
310 320 330 340 350 360
EKEEPQNDAS TTEKNKEKST ESEEYSIDSA KLLTIIRECK SIIEDCKPPS TEEAKKIAAK
370 380 390
NYYCQNPSTL IQKLKRKLNG EDTSSTVEKK QKT
References and
documentation are available.
Number of amino acids: 393
Molecular weight: 45210.45
Theoretical pI: 6.45
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 2014
Hydrogen H 3205
Nitrogen N 531
Oxygen O 625
Sulfur S 11
Formula: C
2014H
3205N
531O
625S
11
Total number of atoms: 6386
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31080
Abs 0.1% (=1 g/l) 0.687, assuming all pairs of Cys residues form cystines
Ext. coefficient 30830
Abs 0.1% (=1 g/l) 0.682, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.93
This classifies the protein as unstable.
Aliphatic index: 81.42
Grand average of hydropathicity (GRAVY): -0.609