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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDIQLNGKS TLDTPSATMS AKEKEAKLKS ADENNKPPNY KRISDDDLYR HSSQYRMWSY

70 80 90 100 110 120
TKDQLQEKRV DTNARAIAYI EENLLKFREA HNLTEEEIKV LEAKAIPLTM EEELDLVNFY

130 140 150 160 170 180
AKKVQVIAQH LNLPTEVVAT AISFFRRFFL ENSVMQIDPK SIVHTTIFLA CKSENYFISV

190 200 210 220 230 240
DSFAQKAKST RDSVLKFEFK LLESLKFSLL NHHPYKPLHG FFLDIQNVLY GKVDLNYMGQ

250 260 270 280 290 300
IYDRCKKRIT AALLTDVVYF YTPPQITLAT LLIEDEALVT RYLETKFPSR EGSQESVPGN

310 320 330 340 350 360
EKEEPQNDAS TTEKNKEKST ESEEYSIDSA KLLTIIRECK SIIEDCKPPS TEEAKKIAAK

370 380 390
NYYCQNPSTL IQKLKRKLNG EDTSSTVEKK QKT


References and documentation are available.

Number of amino acids: 393 Molecular weight: 45210.45 Theoretical pI: 6.45
Amino acid composition: 
Ala (A) 26 6.6% Arg (R) 15 3.8% Asn (N) 20 5.1% Asp (D) 21 5.3% Cys (C) 5 1.3% Gln (Q) 16 4.1% Glu (E) 38 9.7% Gly (G) 7 1.8% His (H) 7 1.8% Ile (I) 24 6.1% Leu (L) 38 9.7% Lys (K) 42 10.7% Met (M) 6 1.5% Phe (F) 16 4.1% Pro (P) 16 4.1% Ser (S) 31 7.9% Thr (T) 29 7.4% Trp (W) 1 0.3% Tyr (Y) 17 4.3% Val (V) 18 4.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 59 Total number of positively charged residues (Arg + Lys): 57 Atomic composition: Carbon C 2014 Hydrogen H 3205 Nitrogen N 531 Oxygen O 625 Sulfur S 11 Formula: C2014H3205N531O625S11 Total number of atoms: 6386 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 31080 Abs 0.1% (=1 g/l) 0.687, assuming all pairs of Cys residues form cystines Ext. coefficient 30830 Abs 0.1% (=1 g/l) 0.682, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.93 This classifies the protein as unstable. Aliphatic index: 81.42 Grand average of hydropathicity (GRAVY): -0.609