ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL HLKKARLDGD 

        70         80         90        100        110        120 
ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG LKKEEGGQGT KQEDLDENSK 

       130        140        150        160        170        180 
QELPMEVPKE PAPAPPPEPD MNNLPQNPIP KHQQKHALLA IKAVKRLKDA RPFLQPVDPV 

       190        200        210        220        230        240 
KLDIPFYFNY IKRPMDLSTI ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA 

       250        260        270        280        290        300 
RNIQASFEKH MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD 

       310        320        330        340        350        360 
IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN LPTYFDYVKE 

       370        380        390        400        410        420 
PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG TIVNMMGHRL EEVFNSKWAD 

       430        440        450        460        470        480 
RPNLDDYDSD EDSRTQGDYD DYESEYSESD IDETIITNPA IQYLEEQLAR MKVELQQLKK 

       490        500        510        520        530        540 
QELEKIRKER RLARGSKKRG KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI 

       550        560        570        580        590        600 
NDLPTSKLER AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG 

       610        620        630        640        650        660 
LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS ASPLSQNGSP 

       670        680 
GQIQSAAHNG FSSSSDDDVS SESEEE 

References and documentation are available.

Number of amino acids: 686

Molecular weight: 76977.95

Theoretical pI: 5.74

Amino acid composition: 
Ala (A)  45	  6.6%
Arg (R)  38	  5.5%
Asn (N)  47	  6.9%
Asp (D)  54	  7.9%
Cys (C)   2	  0.3%
Gln (Q)  35	  5.1%
Glu (E)  54	  7.9%
Gly (G)  37	  5.4%
His (H)  10	  1.5%
Ile (I)  36	  5.2%
Leu (L)  51	  7.4%
Lys (K)  60	  8.7%
Met (M)  18	  2.6%
Phe (F)  16	  2.3%
Pro (P)  45	  6.6%
Ser (S)  59	  8.6%
Thr (T)  29	  4.2%
Trp (W)   2	  0.3%
Tyr (Y)  20	  2.9%
Val (V)  28	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 108
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      3328
Hydrogen    H	      5332
Nitrogen    N	       964
Oxygen      O	      1093
Sulfur      S	        20

Formula: C3328H5332N964O1093S20
Total number of atoms: 10737

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40925
Abs 0.1% (=1 g/l)   0.532, assuming all pairs of Cys residues form cystines


Ext. coefficient    40800
Abs 0.1% (=1 g/l)   0.530, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.89
This classifies the protein as unstable.



Aliphatic index: 67.86

Grand average of hydropathicity (GRAVY): -0.941