ProtParam
User-provided sequence:
10 20 30 40 50 60
MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT PTKEELDAII
70 80 90 100 110 120
EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF RIFDRNADGY IDAEELAEIF
130 140 150 160
RASGEHVTDE EIESLMKDGD KNNDGRIDFD EFLKMMEGVQ
References and
documentation are available.
Number of amino acids: 160
Molecular weight: 18096.01
Theoretical pI: 4.06
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 775
Hydrogen H 1208
Nitrogen N 200
Oxygen O 274
Sulfur S 12
Formula: C
775H
1208N
200O
274S
12
Total number of atoms: 2469
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.165, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.165, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.76
This classifies the protein as unstable.
Aliphatic index: 67.12
Grand average of hydropathicity (GRAVY): -0.569