ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDQRGPPPP HPQQANGYKK FPPHDNQYSG ANNSQPNNHY NENLYSAREP HNNKQYQSKN 

        70         80         90        100        110        120 
GKYGTNKYNN RNNSQGNAQY YNNRFNNGYR LNNNDYNPAM LPGMQWPANY YAPQMYYIPQ 

       130        140        150        160        170        180 
QMVPVASPPY THQPLNTNPE PPSTPKTTKI EITTKTGERL NLKKFHEEKK ASKGEEKNDG 

       190        200        210        220        230        240 
VEQKSKSGTP FEKEATPVLP ANEAVKDTLT ETSNEKSTSE AENTKRLFLE QVRLRKAAME 

       250        260        270        280        290        300 
RKKNGLISET EKKQETSNHD NTDTTKPNSV IESEPIKEAP KPTGEANEVV IDGKSGASVK 

       310        320        330        340        350        360 
TPQHVTGSVT KSVTFNEPEN ESSSQDVDEL VKDDDTTEIS DTTGGKTVNK SDDETINSVI 

       370        380        390        400        410        420 
TTEENTVKET EPSTSDIEMP TVSQLLETLG KAQPISDIYE FAYPENVERP DIKYKKPSVK 

       430        440        450        460        470        480 
YTYGPTFLLQ FKDKLKFRPD PAWVEAVSSK IVIPPHIARN KPKDSGRFGG DFRSPSMRGM 

       490        500        510        520        530        540 
DHTSSSRVSS KRRSKRMGDD RRSNRGYTSR KDREKAAEKA EEQAPKEEIA PLVPSANRWI 

       550        560        570        580        590        600 
PKSRVKKTEK KLAPDGKTEL FDKEEVERKM KSLLNKLTLE MFDSISSEIL DIANQSKWED 

       610        620        630        640        650        660 
DGETLKIVIE QIFHKACDEP HWSSMYAQLC GKVVKDLDPN IKDKENEGKN GPKLVLHYLV 

       670        680        690        700        710        720 
ARCHEEFEKG WADKLPAGED GNPLEPEMMS DEYYIAAAAK RRGLGLVRFI GYLYCLNLLT 

       730        740        750        760        770        780 
GKMMFECFRR LMKDLNNDPS EETLESVIEL LNTVGEQFEH DKFVTPQATL EGSVLLDNLF 

       790        800        810        820        830        840 
MLLQHIIDGG TISNRIKFKL IDVKELREIK HWNSAKKDAG PKTIQQIHQE EEQLRQKKNS 

       850        860        870        880        890        900 
QRSNSRFNNH NQSNSNRYSS NRRNMQNTQR DSFASTKTGS FRNNQRNARK VEEVSQAPRA 

       910 
NMFDALMNND GDSD 

References and documentation are available.

Number of amino acids: 914

Molecular weight: 103898.77

Theoretical pI: 7.30

Amino acid composition: 
Ala (A)  48	  5.3%
Arg (R)  47	  5.1%
Asn (N)  76	  8.3%
Asp (D)  52	  5.7%
Cys (C)   5	  0.5%
Gln (Q)  40	  4.4%
Glu (E)  84	  9.2%
Gly (G)  46	  5.0%
His (H)  19	  2.1%
Ile (I)  37	  4.0%
Leu (L)  56	  6.1%
Lys (K)  89	  9.7%
Met (M)  22	  2.4%
Phe (F)  27	  3.0%
Pro (P)  59	  6.5%
Ser (S)  72	  7.9%
Thr (T)  58	  6.3%
Trp (W)   7	  0.8%
Tyr (Y)  29	  3.2%
Val (V)  41	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 136
Total number of positively charged residues (Arg + Lys): 136

Atomic composition:

Carbon      C	      4510
Hydrogen    H	      7137
Nitrogen    N	      1305
Oxygen      O	      1462
Sulfur      S	        27

Formula: C4510H7137N1305O1462S27
Total number of atoms: 14441

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81960
Abs 0.1% (=1 g/l)   0.789, assuming all pairs of Cys residues form cystines


Ext. coefficient    81710
Abs 0.1% (=1 g/l)   0.786, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.05
This classifies the protein as unstable.



Aliphatic index: 57.94

Grand average of hydropathicity (GRAVY): -1.082