ProtParam
User-provided sequence:
10 20 30 40 50 60
MTDRLTQLQI CLDQMTEQFC ATLNYIDKNH GFERLTVNEP QMSDKHATVV PPEEFSNTID
70 80 90 100 110 120
ELSTDIILKT RQINKLIDSL PGVDVSAEEQ LRKIDMLQKK LVEVEDEKIE AIKKKEKLLR
130 140
HVDSLIEDFV DGIANSKKST
References and
documentation are available.
Number of amino acids: 140
Molecular weight: 16071.37
Theoretical pI: 4.91
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 701
Hydrogen H 1155
Nitrogen N 189
Oxygen O 228
Sulfur S 6
Formula: C
701H
1155N
189O
228S
6
Total number of atoms: 2279
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.100, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.093, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.16
This classifies the protein as unstable.
Aliphatic index: 97.43
Grand average of hydropathicity (GRAVY): -0.506