ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTDSLEYNDI NAEVRGVLCS GRLKMTEQNI IFKNTKTGKV EQISAEDIDL INSQKFVGTW 

        70         80         90        100        110        120 
GLRVFTKGGV LHRFTGFRDS EHEKLGKFIK AAYSQEMVEK EMCVKGWNWG TARFMGSVLS 

       130        140        150        160        170        180 
FDKESKTIFE VPLSHVSQCV TGKNEVTLEF HQNDDAPVGL LEMRFHIPAV ESAEEDPVDK 

       190        200        210        220        230        240 
FHQNVMSKAS VISASGESIA IFREIQILTP RGRYDIKIFS TFFQLHGKTF DYKIPMDSVL 

       250        260        270        280        290        300 
RLFMLPHKDS RQMFFVLSLD PPIKQGQTRY HYLVLLFAPD EETTIELPFS EAELRDKYEG 

       310        320        330        340        350        360 
KLEKEISGPV YEVMGKVMKV LIGRKITGPG NFIGHSGTAA VGCSFKAAAG YLYPLERGFI 

       370        380        390        400        410        420 
YIHKPPLHIR FEEISSVNFA RSGGSTRSFD FEVTLKNGTV HIFSSIEKEE YAKLFDYITQ 

       430        440        450        460        470        480 
KKLHVSNMGK DKSGYKDVDF GDSDNENEPD AYLARLKAEA REKEEDDDDG DSDEESTDED 

       490        500        510        520        530        540 
FKPNENESDV AEEYDSNVES DSDDDSDASG GGGDSDGAKK KKEKKSEKKE KKEKKHKEKE 

       550        560        570        580        590        600 
RTKKPSKKKK DSGKPKRATT AFMLWLNDTR ESIKRENPGI KVTEIAKKGG EMWKELKDKS 

       610        620        630        640        650        660 
KWEDAAAKDK QRYHDEMRNY KPEAGGDSDN EKGGKSSKKR KTEPSPSKKA NTSGSGFKSK 

       670        680        690        700        710        720 
EYISDDDSTS SDDEKDNEPA KKKSKPPSDG DAKKKKAKSE SEPEESEEDS NASDEDEEDE 


ASD 

References and documentation are available.

Number of amino acids: 723

Molecular weight: 81532.82

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  40	  5.5%
Arg (R)  28	  3.9%
Asn (N)  25	  3.5%
Asp (D)  63	  8.7%
Cys (C)   4	  0.6%
Gln (Q)  14	  1.9%
Glu (E)  79	 10.9%
Gly (G)  52	  7.2%
His (H)  16	  2.2%
Ile (I)  34	  4.7%
Leu (L)  37	  5.1%
Lys (K)  92	 12.7%
Met (M)  16	  2.2%
Phe (F)  35	  4.8%
Pro (P)  28	  3.9%
Ser (S)  69	  9.5%
Thr (T)  32	  4.4%
Trp (W)   6	  0.8%
Tyr (Y)  19	  2.6%
Val (V)  34	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 142
Total number of positively charged residues (Arg + Lys): 120

Atomic composition:

Carbon      C	      3572
Hydrogen    H	      5602
Nitrogen    N	       976
Oxygen      O	      1167
Sulfur      S	        20

Formula: C3572H5602N976O1167S20
Total number of atoms: 11337

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61560
Abs 0.1% (=1 g/l)   0.755, assuming all pairs of Cys residues form cystines


Ext. coefficient    61310
Abs 0.1% (=1 g/l)   0.752, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.48
This classifies the protein as stable.



Aliphatic index: 57.47

Grand average of hydropathicity (GRAVY): -0.963