ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEDFISSVK RSNEELKERK SNFGFVEYKS KQLTSSSSHN SNSSHHDDDN QHGKRNIFQR 

        70         80         90        100        110        120 
CVDSFKSPLD GSFDTSNLKR TLKPRHLIMI AIGGSIGTGL FVGSGKAIAE GGPLGVVIGW 

       130        140        150        160        170        180 
AIAGSQIIGT IHGLGEITVR FPVVGAFANY GTRFLDPSIS FVVSTIYVLQ WFFVLPLEII 

       190        200        210        220        230        240 
AAAMTVQYWN SSIDPVIWVA IFYAVIVSIN LFGVRGFGEA EFAFSTIKAI TVCGFIILCV 

       250        260        270        280        290        300 
VLICGGGPDH EFIGAKYWHD PGCLANGFPG VLSVLVVASY SLGGIEMTCL ASGETDPKGL 

       310        320        330        340        350        360 
PSAIKQVFWR ILFFFLISLT LVGFLVPYTN QNLLGGSSVD NSPFVIAIKL HHIKALPSIV 

       370        380        390        400        410        420 
NAVILISVLS VGNSCIFASS RTLCSMAHQG LIPWWFGYID RAGRPLVGIM ANSLFGLLAF 

       430        440        450        460        470        480 
LVKSGSMSEV FNWLMAIAGL ATCIVWLSIN LSHIRFRLAM KAQGKSLDEL EFVSAVGIWG 

       490        500        510        520        530        540 
SAYSALINCL ILIAQFYCSL WPIGGWTSGK ERAKIFFQNY LCALIMLFIF IVHKIYYKCQ 

       550        560        570        580        590 
TGKWWGVKAL KDIDLETDRK DIDIEIVKQE IAEKKMYLDS RPWYVRQFHF WC 

References and documentation are available.

Number of amino acids: 592

Molecular weight: 65404.43

Theoretical pI: 8.63

Amino acid composition: 
Ala (A)  41	  6.9%
Arg (R)  19	  3.2%
Asn (N)  21	  3.5%
Asp (D)  21	  3.5%
Cys (C)  14	  2.4%
Gln (Q)  15	  2.5%
Glu (E)  21	  3.5%
Gly (G)  53	  9.0%
His (H)  14	  2.4%
Ile (I)  61	 10.3%
Leu (L)  57	  9.6%
Lys (K)  30	  5.1%
Met (M)  11	  1.9%
Phe (F)  41	  6.9%
Pro (P)  19	  3.2%
Ser (S)  55	  9.3%
Thr (T)  21	  3.5%
Trp (W)  17	  2.9%
Tyr (Y)  16	  2.7%
Val (V)  45	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      3029
Hydrogen    H	      4660
Nitrogen    N	       760
Oxygen      O	       805
Sulfur      S	        25

Formula: C3029H4660N760O805S25
Total number of atoms: 9279

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118215
Abs 0.1% (=1 g/l)   1.807, assuming all pairs of Cys residues form cystines


Ext. coefficient   117340
Abs 0.1% (=1 g/l)   1.794, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.53
This classifies the protein as stable.



Aliphatic index: 106.71

Grand average of hydropathicity (GRAVY): 0.436