ProtParam
User-provided sequence:
10 20 30 40 50 60
MTEDKSQVKI RFFTREKDEL LHVQDTPMYA PISLKRYGLS EIVNHLLGSE KPVPFDFLIE
70 80 90 100 110 120
GELLRTSLHD YLTKKGLSSE ASLNVEYTRA ILPPSYLNSF SNEDWVSSLD VGDGSKHIIS
130 140 150 160 170 180
GSYDGIVRTW DLSGNVQKQY SGHSGPIRAV KYISNTRLVS AGNDRTLRLW KTKNDDLKLT
190 200 210 220 230 240
SQQQAQEDDD DEVNIEDGKT LAILEGHKAP VVSIDVSDNS RILSASYDNS IGFWSTIYKE
250 260 270 280 290 300
MTVVDPLEDI NNPNNKISTA ARKRRKLTMK DGTIRRRAPL SLLESHTAPV EQVIFDSTDN
310 320 330 340 350 360
TVGYSVSQDH TIKTWDLVTA RCIDTRTTSY SLLSIAQLST LNLLACGSSA RHITLHDPRV
370 380 390 400 410 420
GASSKVTQQQ LIGHKNFVSS LDTCPENEYI LCSGSHDGTV KVWDVRSTSP MYTITREDKS
430 440 450 460
VQKGVNDKVF AVKWAEKVGI ISAGQDKKIQ INKGDNIFKN
References and
documentation are available.
Number of amino acids: 460
Molecular weight: 51358.76
Theoretical pI: 6.47
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2255
Hydrogen H 3603
Nitrogen N 633
Oxygen O 718
Sulfur S 9
Formula: C
2255H
3603N
633O
718S
9
Total number of atoms: 7218
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59610
Abs 0.1% (=1 g/l) 1.161, assuming all pairs of Cys residues form cystines
Ext. coefficient 59360
Abs 0.1% (=1 g/l) 1.156, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.23
This classifies the protein as stable.
Aliphatic index: 87.26
Grand average of hydropathicity (GRAVY): -0.472