ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEDNIAPIT SVKVVTDKCT YKDNELLTKY SYENAVVTKT ASGRFDVTPT VQDYVFKLDL 

        70         80         90        100        110        120 
KKPEKLGIML IGLGGNNGST LVASVLANKH NVEFQTKEGV KQPNYFGSMT QCSTLKLGID 

       130        140        150        160        170        180 
AEGNDVYAPF NSLLPMVSPN DFVVSGWDIN NADLYEAMQR SQVLEYDLQQ RLKAKMSLVK 

       190        200        210        220        230        240 
PLPSIYYPDF IAANQDERAN NCINLDEKGN VTTRGKWTHL QRIRRDIQNF KEENALDKVI 

       250        260        270        280        290        300 
VLWTANTERY VEVSPGVNDT MENLLQSIKN DHEEIAPSTI FAAASILEGV PYINGSPQNT 

       310        320        330        340        350        360 
FVPGLVQLAE HEGTFIAGDD LKSGQTKLKS VLAQFLVDAG IKPVSIASYN HLGNNDGYNL 

       370        380        390        400        410        420 
SAPKQFRSKE ISKSSVIDDI IASNDILYND KLGKKVDHCI VIKYMKPVGD SKVAMDEYYS 

       430        440        450        460        470        480 
ELMLGGHNRI SIHNVCEDSL LATPLIIDLL VMTEFCTRVS YKKVDPVKED AGKFENFYPV 

       490        500        510        520        530 
LTFLSYWLKA PLTRPGFHPV NGLNKQRTAL ENFLRLLIGL PSQNELRFEE RLL 

References and documentation are available.

Number of amino acids: 533

Molecular weight: 59642.06

Theoretical pI: 5.63

Amino acid composition: 
Ala (A)  31	  5.8%
Arg (R)  17	  3.2%
Asn (N)  39	  7.3%
Asp (D)  34	  6.4%
Cys (C)   6	  1.1%
Gln (Q)  19	  3.6%
Glu (E)  32	  6.0%
Gly (G)  31	  5.8%
His (H)   9	  1.7%
Ile (I)  32	  6.0%
Leu (L)  56	 10.5%
Lys (K)  40	  7.5%
Met (M)  11	  2.1%
Phe (F)  20	  3.8%
Pro (P)  25	  4.7%
Ser (S)  35	  6.6%
Thr (T)  29	  5.4%
Trp (W)   4	  0.8%
Tyr (Y)  21	  3.9%
Val (V)  42	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2668
Hydrogen    H	      4214
Nitrogen    N	       704
Oxygen      O	       809
Sulfur      S	        17

Formula: C2668H4214N704O809S17
Total number of atoms: 8412

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53665
Abs 0.1% (=1 g/l)   0.900, assuming all pairs of Cys residues form cystines


Ext. coefficient    53290
Abs 0.1% (=1 g/l)   0.893, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.57
This classifies the protein as stable.



Aliphatic index: 93.06

Grand average of hydropathicity (GRAVY): -0.274