ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR EIVDDSVATA 

        70         80         90        100        110        120 
NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT SMSTSDIPTD LHLEHIGSVS 

       130        140        150        160        170        180 
STNNNSNNAL INHNPLSSHL SNPSSSLRNK KSSLLVASNP AFASDVELSK KKPAVISNNM 

       190        200        210        220        230        240 
PTSNIALYQT ARSANIHGPS STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS 

       250        260        270        280        290        300 
LSQQNKPKII ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH 

       310        320        330        340        350        360 
SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD NTQALTASVS 

       370        380        390        400        410        420 
SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD LSASTSNNNA INDDSHESNS 

       430        440        450        460        470        480 
EKPTKADFFA ARLATAVGEN EISDSEETFV YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ 

       490        500        510        520        530        540 
QQQQQNHGIT SKISAPLLNN NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN 

       550        560        570        580        590        600 
NNNLMSGHNH LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL 

       610        620        630        640        650        660 
VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL RHSSANRNSN 

       670        680        690        700        710        720 
YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY IEQPHNYPTM QNSVKKDEFY 

       730        740        750        760        770        780 
NSRNNKFPHG LNFYGDNNVI EEENNGDSSN VNRPQHTNLQ HEFIPEDNES DENDIHSMFY 

       790        800        810        820        830        840 
YNHKNDLETK PLISDYGEDE DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY 

       850        860        870        880        890        900 
DFMVGNNTGN NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV 

       910        920        930        940        950        960 
GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV DVVVMDNVIS 

       970        980        990       1000       1010       1020 
SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD SNGDCTVMEQ ERKILQITTN 

      1030       1040       1050       1060       1070       1080 
LSLVEESANN DISGGNIETV LLGTAKKLET PLKFQGGAFN RNYDVSVSSV KLLSPGSREA 

      1090       1100       1110       1120       1130       1140 
KHENDDDDDD DGDDGDDENN TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK 

      1150       1160 
YEVPFFNTQK STAIQKDSMV HPGKK 

References and documentation are available.

Number of amino acids: 1165

Molecular weight: 128140.50

Theoretical pI: 5.59

Amino acid composition: 
Ala (A)  63	  5.4%
Arg (R)  36	  3.1%
Asn (N) 161	 13.8%
Asp (D)  85	  7.3%
Cys (C)   4	  0.3%
Gln (Q)  43	  3.7%
Glu (E)  54	  4.6%
Gly (G)  44	  3.8%
His (H)  38	  3.3%
Ile (I)  55	  4.7%
Leu (L)  84	  7.2%
Lys (K)  70	  6.0%
Met (M)  21	  1.8%
Phe (F)  30	  2.6%
Pro (P)  63	  5.4%
Ser (S) 154	 13.2%
Thr (T)  77	  6.6%
Trp (W)   2	  0.2%
Tyr (Y)  26	  2.2%
Val (V)  55	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 139
Total number of positively charged residues (Arg + Lys): 106

Atomic composition:

Carbon      C	      5447
Hydrogen    H	      8607
Nitrogen    N	      1625
Oxygen      O	      1905
Sulfur      S	        25

Formula: C5447H8607N1625O1905S25
Total number of atoms: 17609

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49990
Abs 0.1% (=1 g/l)   0.390, assuming all pairs of Cys residues form cystines


Ext. coefficient    49740
Abs 0.1% (=1 g/l)   0.388, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.09
This classifies the protein as unstable.



Aliphatic index: 65.63

Grand average of hydropathicity (GRAVY): -0.895