ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEEDSSAKM SEDVEKYLNL NPPCSSSSSS SSAATFTNKS RNFKSSPPPC PDHVLENVLE 

        70         80         90        100        110        120 
NVLQFLTSRC DRNAVSLVCR SWYRVEAQTR LEVFIGNCYS LSPARLIHRF KRVRSLVLKG 

       130        140        150        160        170        180 
KPRFADFNLM PPNWGAQFSP WVAATAKAYP WLEKVHLKRM FVTDDDLALL AESFPGFKEL 

       190        200        210        220        230        240 
TLVCCEGFGT SGIAIVANKC RQLKVLDLME SEVTDDELDW ISCFPEGETH LESLSFDCVE 

       250        260        270        280        290        300 
SPINFKALEE LVVRSPFLKK LRTNRFVSLE ELHRLMVRAP QLTSLGTGSF SPDNVPQGEQ 

       310        320        330        340        350        360 
QPDYAAAFRA CKSIVCLSGF REFRPEYLLA ISSVCANLTS LNFSYANISP HMLKPIISNC 

       370        380        390        400        410        420 
HNIRVFWALD SIRDEGLQAV AATCKELREL RIFPFDPRED SEGPVSGVGL QAISEGCRKL 

       430        440        450        460        470        480 
ESILYFCQNM TNGAVTAMSE NCPQLTVFRL CIMGRHRPDH VTGKPMDDGF GAIVKNCKKL 

       490        500        510        520        530        540 
TRLAVSGLLT DEAFSYIGEY GKLIRTLSVA FAGNSDKALR YVLEGCPKLQ KLEIRDSPFG 

       550        560        570        580        590        600 
DVGLRSGMHR YSNMRFVWLS SCLISRGGCR GVSHALPNVV VEVFGADGDD DEDTVTGDYV 

       610        620 
ETLYLYRSLD GPRKDAPKFV TIL 

References and documentation are available.

Number of amino acids: 623

Molecular weight: 69499.63

Theoretical pI: 6.31

Amino acid composition: 
Ala (A)  41	  6.6%
Arg (R)  41	  6.6%
Asn (N)  26	  4.2%
Asp (D)  34	  5.5%
Cys (C)  23	  3.7%
Gln (Q)  13	  2.1%
Glu (E)  41	  6.6%
Gly (G)  37	  5.9%
His (H)  11	  1.8%
Ile (I)  23	  3.7%
Leu (L)  68	 10.9%
Lys (K)  30	  4.8%
Met (M)  13	  2.1%
Phe (F)  34	  5.5%
Pro (P)  34	  5.5%
Ser (S)  59	  9.5%
Thr (T)  27	  4.3%
Trp (W)   7	  1.1%
Tyr (Y)  15	  2.4%
Val (V)  46	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      3082
Hydrogen    H	      4844
Nitrogen    N	       844
Oxygen      O	       914
Sulfur      S	        36

Formula: C3082H4844N844O914S36
Total number of atoms: 9720

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62225
Abs 0.1% (=1 g/l)   0.895, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   0.876, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.21
This classifies the protein as unstable.



Aliphatic index: 84.96

Grand average of hydropathicity (GRAVY): -0.145