ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEFYSDTIG LPKTDPRLWR LRTDELGRES WEYLTPQQAA NDPPSTFTQW LLQDPKFPQP 

        70         80         90        100        110        120 
HPERNKHSPD FSAFDACHNG ASFFKLLQEP DSGIFPCQYK GPMFMTIGYV AVNYIAGIEI 

       130        140        150        160        170        180 
PEHERIELIR YIVNTAHPVD GGWGLHSVDK STVFGTVLNY VILRLLGLPK DHPVCAKARS 

       190        200        210        220        230        240 
TLLRLGGAIG SPHWGKIWLS ALNLYKWEGV NPAPPETWLL PYSLPMHPGR WWVHTRGVYI 

       250        260        270        280        290        300 
PVSYLSLVKF SCPMTPLLEE LRNEIYTKPF DKINFSKNRN TVCGVDLYYP HSTTLNIANS 

       310        320        330        340        350        360 
LVVFYEKYLR NRFIYSLSKK KVYDLIKTEL QNTDSLCIAP VNQAFCALVT LIEEGVDSEA 

       370        380        390        400        410        420 
FQRLQYRFKD ALFHGPQGMT IMGTNGVQTW DCAFAIQYFF VAGLAERPEF YNTIVSAYKF 

       430        440        450        460        470        480 
LCHAQFDTEC VPGSYRDKRK GAWGFSTKTQ GYTVADCTAE AIKAIIMVKN SPVFSEVHHM 

       490        500        510        520        530        540 
ISSERLFEGI DVLLNLQNIG SFEYGSFATY EKIKAPLAME TLNPAEVFGN IMVEYPYVEC 

       550        560        570        580        590        600 
TDSSVLGLTY FHKYFDYRKE EIRTRIRIAI EFIKKSQLPD GSWYGSWGIC FTYAGMFALE 

       610        620        630        640        650        660 
ALHTVGETYE NSSTVRKGCD FLVSKQMKDG GWGESMKSSE LHSYVDSEKS LVVQTAWALI 

       670        680        690        700        710        720 
ALLFAEYPNK EVIDRGIDLL KNRQEESGEW KFESVEGVFN HSCAIEYPSY RFLFPIKALG 

       730 
MYSRAYETHT L 

References and documentation are available.

Number of amino acids: 731

Molecular weight: 83460.42

Theoretical pI: 6.16

Amino acid composition: 
Ala (A)  45	  6.2%
Arg (R)  31	  4.2%
Asn (N)  28	  3.8%
Asp (D)  30	  4.1%
Cys (C)  15	  2.1%
Gln (Q)  21	  2.9%
Glu (E)  51	  7.0%
Gly (G)  48	  6.6%
His (H)  20	  2.7%
Ile (I)  42	  5.7%
Leu (L)  66	  9.0%
Lys (K)  41	  5.6%
Met (M)  15	  2.1%
Phe (F)  42	  5.7%
Pro (P)  41	  5.6%
Ser (S)  51	  7.0%
Thr (T)  43	  5.9%
Trp (W)  17	  2.3%
Tyr (Y)  40	  5.5%
Val (V)  44	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3818
Hydrogen    H	      5763
Nitrogen    N	       971
Oxygen      O	      1077
Sulfur      S	        30

Formula: C3818H5763N971O1077S30
Total number of atoms: 11659

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   153975
Abs 0.1% (=1 g/l)   1.845, assuming all pairs of Cys residues form cystines


Ext. coefficient   153100
Abs 0.1% (=1 g/l)   1.834, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.76
This classifies the protein as stable.



Aliphatic index: 81.23

Grand average of hydropathicity (GRAVY): -0.208