ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEHESLGLE QNQDGGDTYR HHNLSDGCIS SVEDANEQPS SYEEESDDDM QYYERAIQEI 

        70         80         90        100        110        120 
SSGDSYVCMI CTVEMDYTCQ MFACKRCYRV FDYGCIREWA LKSTEKTVDR IWKCPNCYYV 

       130        140        150        160        170        180 
SKRVPVKNRP TCWCGKVVNP DPNPLDPNSC GQTCNASTCM HGCSKICHLG PHPECTRMVE 

       190        200        210        220        230        240 
IMCHCGKHSK SIFCYQSKVM KKNFNCQEVC GLPLSCSIHT CKKKCHPGLC GPCPEMIISK 

       250        260        270        280        290        300 
DSPKKQIKCY CGNHTRANIK CSETKFPKSG KSSKDENGNR WIGVFACADN RVVDYSCRKH 

       310        320        330        340        350        360 
SFIESCISPP TINGEKACPF LPSSLKTCPC GRTALEELTK PRKHCDDPIP TCDSRCGKPL 

       370        380        390        400        410        420 
KCGKHSCPFT CHDKACMEPC LQIDSVKCAC EQSTFSVPCG FQGRPRCNIK CESLMSCRRH 

       430        440        450        460        470        480 
RCTDRCCSGR PSAIRRKKNL FRTQDLLDES LVEAKHICLK PCNLTLSCGI HKCQRKCHPG 

       490        500        510        520        530        540 
KCPPCLESDS NDLVCPCGNT VVPAPVRCGT KLPTCNHPCI KVVRGESTCG HKPMPHTCHS 

       550        560        570        580        590        600 
LDVSCPPCTE TVFKPCKCGK KTKVRTVCFQ TDVSCGIKCG IPLSYCYHTC QKTCHLPGNC 

       610        620        630        640        650        660 
QKVCKQTCGQ KRLNCNHECP KPCHGKTECP DLPCATLVKI YCKCGRIKKS VTCGAKSDRV 

       670        680        690        700        710        720 
SVTESSVLDC NEECEALKRL KELKEAFGIK EETNNFTSNE LDALKKLVSV ATTFEELQLP 

       730        740        750        760        770        780 
FTEAALSVYS KQERWCSQIE AILNKLMDDK TRSSLHFKPM RPPQRHFIRE LAKAYGLYSE 

       790        800        810        820        830        840 
SQDREPMRSV FIKKEDNGAS NKPVLSLAEA YPLYESFKQL QKERKAQEFQ ARTTAKLINF 

       850        860        870        880        890        900 
EVQDTEPKVE VAKKNGFLVQ NLVAGNTAED LRRFFEPHLK HTLVVNPQYL ILDDGKTALV 

       910        920        930        940        950        960 
YPENYETASV NTERDMELLV GHFDFMAKEA FLADSISLCS TEEELERRLD TPVIQEDSPV 


MDNNT 

References and documentation are available.

Number of amino acids: 965

Molecular weight: 108494.81

Theoretical pI: 8.29

Amino acid composition: 
Ala (A)  40	  4.1%
Arg (R)  48	  5.0%
Asn (N)  42	  4.4%
Asp (D)  47	  4.9%
Cys (C)  92	  9.5%
Gln (Q)  33	  3.4%
Glu (E)  70	  7.3%
Gly (G)  46	  4.8%
His (H)  32	  3.3%
Ile (I)  38	  3.9%
Leu (L)  66	  6.8%
Lys (K)  87	  9.0%
Met (M)  19	  2.0%
Phe (F)  30	  3.1%
Pro (P)  63	  6.5%
Ser (S)  73	  7.6%
Thr (T)  57	  5.9%
Trp (W)   5	  0.5%
Tyr (Y)  24	  2.5%
Val (V)  53	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 117
Total number of positively charged residues (Arg + Lys): 135

Atomic composition:

Carbon      C	      4648
Hydrogen    H	      7418
Nitrogen    N	      1340
Oxygen      O	      1429
Sulfur      S	       111

Formula: C4648H7418N1340O1429S111
Total number of atoms: 14946

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69010
Abs 0.1% (=1 g/l)   0.636, assuming all pairs of Cys residues form cystines


Ext. coefficient    63260
Abs 0.1% (=1 g/l)   0.583, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.95
This classifies the protein as unstable.



Aliphatic index: 62.10

Grand average of hydropathicity (GRAVY): -0.535