ProtParam
User-provided sequence:
10 20 30 40 50 60
MTEPAQKKQK QTVQERKAFI SRITNETKIQ IAISLNGGYI QIKDSILPAK KDDDVASQAT
70 80 90 100 110 120
QSQVIDIHTG VGFLDHMIHA LAKHSGWSLI VECIGDLHID DHHTTEDCGI ALGQAFKEAM
130 140 150 160 170 180
GAVRGVKRFG TGFAPLDEAL SRAVVDLSNR PFAVIDLGLK REMIGDLSTE MIPHFLESFA
190 200 210 220 230
EAARITLHVD CLRGFNDHHR SESAFKALAV AIREAISSNG TNDVPSTKGV LM
References and
documentation are available.
Number of amino acids: 232
Molecular weight: 25324.90
Theoretical pI: 6.11
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1112
Hydrogen H 1788
Nitrogen N 318
Oxygen O 339
Sulfur S 9
Formula: C
1112H
1788N
318O
339S
9
Total number of atoms: 3566
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7115
Abs 0.1% (=1 g/l) 0.281, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 0.276, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.31
This classifies the protein as stable.
Aliphatic index: 93.41
Grand average of hydropathicity (GRAVY): -0.125