ProtParam
User-provided sequence:
10 20 30 40 50 60
MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW
70 80 90 100 110 120
RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF
130 140 150 160 170 180
YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF
190 200 210 220 230 240
YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV
250 260
DPNAGDGEPK EQIQDVEDQD VS
References and
documentation are available.
Number of amino acids: 262
Molecular weight: 29798.53
Theoretical pI: 4.68
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1300
Hydrogen H 2067
Nitrogen N 351
Oxygen O 425
Sulfur S 12
Formula: C
1300H
2067N
351O
425S
12
Total number of atoms: 4155
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29005
Abs 0.1% (=1 g/l) 0.973, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.969, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.59
This classifies the protein as stable.
Aliphatic index: 83.85
Grand average of hydropathicity (GRAVY): -0.550