ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTETHHAPLP DVYPSSKQPT SSTYSKCKKF GLPLIGLLTL LLAYISSFTK PVPNSTFDVP 

        70         80         90        100        110        120 
ASPQCKKPQV YRPSFNKSVN LILNDKQFKI DSIRKLSGAI QIPTEISDTN PLPNDDPEYY 

       130        140        150        160        170        180 
SEFFKLHKYF ETFPLVHSHL KVEKVNQLGL LYTWEGTDPS LKPILFMAHQ DVVPVNREIW 

       190        200        210        220        230        240 
DSWQYPPLSG HYDQETDYVW GRGSNDCKNL MLAELEGIEQ LLADGYQTER TVILSLGFDE 

       250        260        270        280        290        300 
ESSGFMGAKV LAPFLLERYG PDSMFSIIDE GAGLLRLDKN LYIAAAVNAE KGYVDVRISI 

       310        320        330        340        350        360 
HGHGGHSSVQ PDHTTIGVAS ELIYMMENHP FDYNFSLDNP IYDVLQCAAE HSGFLPPHVR 

       370        380        390        400        410        420 
EAILKAPVDE GKRKVLTEFA ASHPDIRDLI RTTRAVDVIN GGVKANALPG LTSFIVNHRV 

       430        440        450        460        470        480 
DIHSSVNETV NDLYWARVIA EKHGYGLTFH DEIIIPETKL GHISLASEKM LEPAPVSPTS 

       490        500        510        520        530        540 
GHVWEIFAGT VQNLFQNEIL AEQKDADVYV TGGLFSGNTD TKYYWGLSKN IYRFVAGIFP 

       550        560        570 
FDQLRTIHSV NEHISASSHV SAVAFVYEYI VNVNEYGHD 

References and documentation are available.

Number of amino acids: 579

Molecular weight: 64834.34

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  35	  6.0%
Arg (R)  17	  2.9%
Asn (N)  27	  4.7%
Asp (D)  35	  6.0%
Cys (C)   4	  0.7%
Gln (Q)  17	  2.9%
Glu (E)  36	  6.2%
Gly (G)  37	  6.4%
His (H)  25	  4.3%
Ile (I)  38	  6.6%
Leu (L)  54	  9.3%
Lys (K)  30	  5.2%
Met (M)   8	  1.4%
Phe (F)  27	  4.7%
Pro (P)  36	  6.2%
Ser (S)  46	  7.9%
Thr (T)  30	  5.2%
Trp (W)   7	  1.2%
Tyr (Y)  27	  4.7%
Val (V)  43	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      2944
Hydrogen    H	      4491
Nitrogen    N	       761
Oxygen      O	       869
Sulfur      S	        12

Formula: C2944H4491N761O869S12
Total number of atoms: 9077

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78980
Abs 0.1% (=1 g/l)   1.218, assuming all pairs of Cys residues form cystines


Ext. coefficient    78730
Abs 0.1% (=1 g/l)   1.214, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.57
This classifies the protein as stable.



Aliphatic index: 89.55

Grand average of hydropathicity (GRAVY): -0.219