ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTEVLLLPGT KSENSKPPHI VVFPFPAQGH LLPLLDLTHQ LCLRGFNVSV IVTPGNLTYL 

        70         80         90        100        110        120 
SPLLSAHPSS VTSVVFPFPP HPSLSPGVEN VKDVGNSGNL PIMASLRQLR EPIINWFQSH 

       130        140        150        160        170        180 
PNPPIALISD FFLGWTHDLC NQIGIPRFAF FSISFFLVSV LQFCFENIDL IKSTDPIHLL 

       190        200        210        220        230        240 
DLPRAPIFKE EHLPSIVRRS LQTPSPDLES IKDFSMNLLS YGSVFNSSEI LEDDYLQYVK 

       250        260        270        280        290        300 
QRMGHDRVYV IGPLCSIGSG LKSNSGSVDP SLLSWLDGSP NGSVLYVCFG SQKALTKDQC 

       310        320        330        340        350        360 
DALALGLEKS MTRFVWVVKK DPIPDGFEDR VSGRGLVVRG WVSQLAVLRH VAVGGFLSHC 

       370        380        390        400        410        420 
GWNSVLEGIT SGAVILGWPM EADQFVNARL LVEHLGVAVR VCEGGETVPD SDELGRVIAE 

       430        440        450        460 
TMGEGGREVA ARAEEIRRKT EAAVTEANGS SVENVQRLVK EFEKV 

References and documentation are available.

Number of amino acids: 465

Molecular weight: 50942.50

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  22	  4.7%
Arg (R)  22	  4.7%
Asn (N)  18	  3.9%
Asp (D)  22	  4.7%
Cys (C)   8	  1.7%
Gln (Q)  14	  3.0%
Glu (E)  28	  6.0%
Gly (G)  37	  8.0%
His (H)  13	  2.8%
Ile (I)  24	  5.2%
Leu (L)  55	 11.8%
Lys (K)  16	  3.4%
Met (M)   7	  1.5%
Phe (F)  24	  5.2%
Pro (P)  33	  7.1%
Ser (S)  46	  9.9%
Thr (T)  16	  3.4%
Trp (W)   7	  1.5%
Tyr (Y)   6	  1.3%
Val (V)  47	 10.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      2298
Hydrogen    H	      3604
Nitrogen    N	       612
Oxygen      O	       666
Sulfur      S	        15

Formula: C2298H3604N612O666S15
Total number of atoms: 7195

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47940
Abs 0.1% (=1 g/l)   0.941, assuming all pairs of Cys residues form cystines


Ext. coefficient    47440
Abs 0.1% (=1 g/l)   0.931, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.31
This classifies the protein as stable.



Aliphatic index: 100.30

Grand average of hydropathicity (GRAVY): 0.075