ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTFNSFEGSR TVVPADTNKD EEFVEEFNRL KTFANFPSSS PVSASTLARA GFLYTGEGDT 

        70         80         90        100        110        120 
VQCFSCHAAV DRWQYGDSAV GRHRRISPNC RFINGFYFEN GATQSTSPGI QNGQYKSENC 

       130        140        150        160        170        180 
VGNRNHFALD RPSETHADYL LRTGQVVDIS DTIYPRNPAM CSEEARPKTF QNWPDYAHLS 

       190        200        210        220        230        240 
PRELASAGLY YTGIDDQVQC FCCGGKLKNW EPCDRAWSEH RRHFPNCFFV LGRNVNVRSE 

       250        260        270        280        290        300 
SGVSSDRNFP NSTNSPRNPA MAEYDARIVT FGTWLYSVNK EQLARAGFYA LGEGDKVKCF 

       310        320        330        340        350        360 
HCGGGLTDWK PSEDPWEQHA KWYPGCKYLL DEKGQEYINN IHLTHSLGES VVRTAEKTPS 

       370        380        390        400        410        420 
VTKKIDDTIF QNPMVQEAIR MGFNFKDIKK TMEEKLQTSG SNYLSLEVLI ADLVSAQKDN 

       430        440        450        460        470        480 
SQDESSQTSL QKDISTEEQL RRLQEEKLCK ICMDRNIAIV FVPCGHLVTC KQCAEAVDKC 

       490 
PMCCTVITFK QKIFMF 

References and documentation are available.

Number of amino acids: 496

Molecular weight: 56116.97

Theoretical pI: 6.10

Amino acid composition: 
Ala (A)  31	  6.2%
Arg (R)  28	  5.6%
Asn (N)  29	  5.8%
Asp (D)  28	  5.6%
Cys (C)  21	  4.2%
Gln (Q)  23	  4.6%
Glu (E)  34	  6.9%
Gly (G)  31	  6.2%
His (H)  12	  2.4%
Ile (I)  20	  4.0%
Leu (L)  29	  5.8%
Lys (K)  28	  5.6%
Met (M)   9	  1.8%
Phe (F)  28	  5.6%
Pro (P)  23	  4.6%
Ser (S)  39	  7.9%
Thr (T)  30	  6.0%
Trp (W)   8	  1.6%
Tyr (Y)  16	  3.2%
Val (V)  29	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2459
Hydrogen    H	      3770
Nitrogen    N	       692
Oxygen      O	       758
Sulfur      S	        30

Formula: C2459H3770N692O758S30
Total number of atoms: 7709

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69090
Abs 0.1% (=1 g/l)   1.231, assuming all pairs of Cys residues form cystines


Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.209, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.31
This classifies the protein as unstable.



Aliphatic index: 61.73

Grand average of hydropathicity (GRAVY): -0.557