ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTFTSNLPSS SEQSISPPAS SFSSSTDTLK DIDIPHNGAD LSTYSKFLAL YCRSDKCDDF 

        70         80         90        100        110        120 
YSLEEKQNCK FGDQWLDFIN TIHNLDFSES EVSGRVSERI LPASLANKFT NNLGVAIKIS 

       130        140        150        160        170        180 
EYTRDDERQI RGCVTTVENE NSFNNWFVYH ILDQSQLSLS EHPIVTKEVK YHELFADFFE 

       190        200        210        220        230        240 
KNLKNTIVND QWNFGGRDYF IERSRYFTDR YLRIECILPA FPCKSSNEQK VYGSVPDKGE 

       250        260        270        280        290        300 
ELALKRLIKA TQDLVKIYPP GMKIWIVSDG HVFSDCIGVD DDVVSTYTTK LHELYKRVAI 

       310        320        330        340        350        360 
PGVDAIGFCG LNDLFFSGAA SKVFDPKWVS DVEVAHYTGT QICPKSDLSR QILMKGCDTD 

       370        380        390        400        410        420 
AGRLRKQIAI EGHPRLHLYR GFSRFMMEDL SLLEHFQSYS RKKFKKIISM IAFNMIKRND 

       430        440        450        460        470        480 
AYSNLVELIF PHHLRISIHA HTNSGPKFGI KVISNEQCSI VSSLEDLDEP KFEDFLHIPT 

       490        500        510        520        530 
PWHNCVVKVE DEKEKYFLTK SKVVKEALEK GMYDGVWKDT RFDIGEGGHF VIKKIS 

References and documentation are available.

Number of amino acids: 536

Molecular weight: 61390.71

Theoretical pI: 6.08

Amino acid composition: 
Ala (A)  21	  3.9%
Arg (R)  23	  4.3%
Asn (N)  24	  4.5%
Asp (D)  40	  7.5%
Cys (C)  12	  2.2%
Gln (Q)  14	  2.6%
Glu (E)  35	  6.5%
Gly (G)  28	  5.2%
His (H)  18	  3.4%
Ile (I)  40	  7.5%
Leu (L)  42	  7.8%
Lys (K)  43	  8.0%
Met (M)   8	  1.5%
Phe (F)  34	  6.3%
Pro (P)  20	  3.7%
Ser (S)  51	  9.5%
Thr (T)  24	  4.5%
Trp (W)   7	  1.3%
Tyr (Y)  19	  3.5%
Val (V)  33	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2760
Hydrogen    H	      4251
Nitrogen    N	       729
Oxygen      O	       819
Sulfur      S	        20

Formula: C2760H4251N729O819S20
Total number of atoms: 8579

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67560
Abs 0.1% (=1 g/l)   1.100, assuming all pairs of Cys residues form cystines


Ext. coefficient    66810
Abs 0.1% (=1 g/l)   1.088, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.65
This classifies the protein as stable.



Aliphatic index: 81.44

Grand average of hydropathicity (GRAVY): -0.373