ProtParam
User-provided sequence:
10 20 30 40 50 60
MTGDSRSISE PSINLDPDNT SFSDENSDDF FMDNSYDIDE IDHSDESNRQ SVIVDSKVTV
70 80 90 100 110 120
PPSKHSTLTL SDSEDSDAKE QHQSLSRSSS KNVNIEDITE PKPDKPSGRT RGRSVMKESV
130 140 150 160 170 180
VEINSSESDL DEDKNFPRSR SRSRSSIRSI SPAGKYKRQK SSLLYTYDEN DDFFKELAKE
190 200 210 220 230 240
AKKSTTISKE STPDQRKRVY NIKFLSKLEG TINKAVQVKV LGKYEFSKIL PAALDGLMKS
250 260 270 280 290 300
YKIPKVMKDI YKVENVTLYW NNAKLLTFMT CNSLHIPQDF ENEVSDIDVT IVSKEYEKNF
310 320 330 340 350 360
EATLESKLKE EEAALLIKER QEMERKLEKK RNEQEESEYR EFESELKNVE ETQEIKENDT
370 380 390 400 410 420
VMNTKLLQEG GSLSGNSSSM EEVMRIALMG QDNKKIYVHV RRSTPFSKIA EYYRIQKQLP
430 440 450
QKTRVKLLFD HDELDMNECI ADQDMEDEDM VDVIID
References and
documentation are available.
Number of amino acids: 456
Molecular weight: 52541.55
Theoretical pI: 4.96
Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 2269
Hydrogen H 3641
Nitrogen N 625
Oxygen O 772
Sulfur S 16
Formula: C
2269H
3641N
625O
772S
16
Total number of atoms: 7323
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26485
Abs 0.1% (=1 g/l) 0.504, assuming all pairs of Cys residues form cystines
Ext. coefficient 26360
Abs 0.1% (=1 g/l) 0.502, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.22
This classifies the protein as unstable.
Aliphatic index: 70.92
Grand average of hydropathicity (GRAVY): -0.919