ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL DSTEVSNLVQ 

        70         80         90        100        110        120 
VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD CDLIYTYSGL FLVAINPYHN 

       130        140        150        160        170        180 
LNLYSEDHIN LYHNKHNRLS KSRLDENSHE KLPPHIFAIA EEAYENLLSE GKDQSILVTG 

       190        200        210        220        230        240 
ESGAGKTENT KKILQYLASI TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV 

       250        260        270        280        290        300 
RNNNSSRFGK FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS 

       310        320        330        340        350        360 
ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD QIRWIFQVVA 

       370        380        390        400        410        420 
IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT AILRPRSKAG KEWVSQSKNS 

       430        440        450        460        470        480 
QQAKFILNAL SRNLYERLFG YIVDMINKNL DHGSATLNYI GLLDIAGFEI FENNSFEQLC 

       490        500        510        520        530        540 
INYTNEKLQQ FFNNHMFVLE QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL 

       550        560        570        580        590        600 
DEEAVLPKST DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD 

       610        620        630        640        650        660 
PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS TFKTTSSRHR 

       670        680        690        700        710        720 
EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ LRCNGVLEGI RLAREGYPNR 

       730        740        750        760        770        780 
IAFQEFFQRY RILYPENSTT TTFSSKLKAS TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA 

       790        800        810        820        830        840 
GVLADLEKQK DVKLNNIMIK LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE 

       850        860        870        880        890        900 
DPWFNLFIRI KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN 

       910        920        930        940        950        960 
TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV AQNLEEAHQK 

       970        980        990       1000       1010       1020 
IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ SSQSLIKESK LKLENEIKRL 

      1030       1040       1050       1060       1070       1080 
KDVINSKEEE IKSFNDKLSS SEEDLDIKLV TLEKNCNIAM SRLQSLVTEN SDLRSKNENF 

      1090       1100       1110       1120       1130       1140 
KKEKAALNNQ LKNKESELLK MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL 

      1150       1160       1170       1180       1190       1200 
TEYKSNYQKI KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK 

      1210       1220       1230       1240       1250       1260 
RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN EIEEKKNLIS 

      1270       1280       1290       1300       1310       1320 
RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD SILNEPLDNC PDKESDINKL 

      1330       1340       1350       1360       1370       1380 
MLEVDYLKRQ LDIETRAHYD AENAISALHS KFRKIQGESS LSSSDIYKLK FEASEERVKS 

      1390       1400       1410       1420       1430       1440 
LEDKLKTMPL RDRTNLPVGD IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT 

      1450       1460       1470       1480       1490       1500 
LDLRQSKSKE ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR 

      1510       1520       1530       1540       1550       1560 
EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE TLLELKRVQD 

      1570       1580       1590       1600       1610       1620 
VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL KAETNLKKEF ATLKYKLETS 

      1630       1640       1650       1660       1670       1680 
TNDSEAKISD LLKQLDHYTK VVEMLNNEKD AISLAEKELY QKYEALNTEC ESLKGKIVSL 

      1690       1700       1710       1720       1730       1740 
TKIKQELESD LNQKTDALQI SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV 

      1750       1760       1770       1780       1790       1800 
KTLQASCNGY KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS 

      1810       1820       1830       1840       1850       1860 
KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN RSEFEEATLK 

      1870       1880       1890       1900       1910       1920 
YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI EFWKSRYEST MIGSKNIDSN 


NAQSKIFS 

References and documentation are available.

Number of amino acids: 1928

Molecular weight: 223635.76

Theoretical pI: 6.06

Amino acid composition: 
Ala (A)  73	  3.8%
Arg (R)  83	  4.3%
Asn (N) 151	  7.8%
Asp (D) 107	  5.5%
Cys (C)  14	  0.7%
Gln (Q) 110	  5.7%
Glu (E) 204	 10.6%
Gly (G)  52	  2.7%
His (H)  31	  1.6%
Ile (I) 133	  6.9%
Leu (L) 229	 11.9%
Lys (K) 208	 10.8%
Met (M)  28	  1.5%
Phe (F)  61	  3.2%
Pro (P)  32	  1.7%
Ser (S) 182	  9.4%
Thr (T)  94	  4.9%
Trp (W)  11	  0.6%
Tyr (Y)  57	  3.0%
Val (V)  68	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 311
Total number of positively charged residues (Arg + Lys): 291

Atomic composition:

Carbon      C	      9816
Hydrogen    H	     15861
Nitrogen    N	      2719
Oxygen      O	      3145
Sulfur      S	        42

Formula: C9816H15861N2719O3145S42
Total number of atoms: 31583

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   146305
Abs 0.1% (=1 g/l)   0.654, assuming all pairs of Cys residues form cystines


Ext. coefficient   145430
Abs 0.1% (=1 g/l)   0.650, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.77
This classifies the protein as unstable.



Aliphatic index: 87.24

Grand average of hydropathicity (GRAVY): -0.782