ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTGKRSKTNC RSASHKLFKD KAKNRVDDLQ GMLLDLQFAR KESRPTDVTL LEEQVNQMLR 

        70         80         90        100        110        120 
EWKSELNEPS PASSLQQGGT LGSFSSDICR LLQLCDEEDD ATSKLAAPKP EPADQNLEAG 

       130        140        150        160        170        180 
KAAVFQRGYN LVQGKSEHGL PLVDNCKDLS LAAGNNFDGT APLEYHQQYD LQQEFEPNFN 

       190        200        210        220        230        240 
GGFNNCPSYG VVEGPIHISN FIPTICPPPS AFLGPKCALW DCPRPAQGFD WFQDYCSSFH 

       250        260        270        280        290        300 
AALAFNEGPP GMNPVVRPGG IGLKDGLLFA ALSAKAGGKD VGIPECEGAA TAKSPWNAPE 

       310        320        330        340        350        360 
LFDLTVLESE TLREWLFFDK PRRAFESGNR KQRSLPDYNG RGWHESRKQI MVEFGGLKRS 

       370        380        390        400        410        420 
YYMDPQPLHH FEWHLYEYEI NKCDACALYR LELKLVDGKK TSKGKVSNDS VADLQKQMGR 

       430        440        450        460        470        480 
LTAEFPPENN TTNTTNNNKR CIKGRPKVST KVATGNVQNT VEQANDYGVG EEFNYLVGNL 


SDYYIP 

References and documentation are available.

Number of amino acids: 486

Molecular weight: 54079.70

Theoretical pI: 5.73

Amino acid composition: 
Ala (A)  35	  7.2%
Arg (R)  22	  4.5%
Asn (N)  32	  6.6%
Asp (D)  29	  6.0%
Cys (C)  13	  2.7%
Gln (Q)  23	  4.7%
Glu (E)  34	  7.0%
Gly (G)  40	  8.2%
His (H)   9	  1.9%
Ile (I)  11	  2.3%
Leu (L)  47	  9.7%
Lys (K)  33	  6.8%
Met (M)   7	  1.4%
Phe (F)  23	  4.7%
Pro (P)  33	  6.8%
Ser (S)  31	  6.4%
Thr (T)  20	  4.1%
Trp (W)   7	  1.4%
Tyr (Y)  15	  3.1%
Val (V)  22	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2387
Hydrogen    H	      3681
Nitrogen    N	       665
Oxygen      O	       734
Sulfur      S	        20

Formula: C2387H3681N665O734S20
Total number of atoms: 7487

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61600
Abs 0.1% (=1 g/l)   1.139, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   1.125, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.59
This classifies the protein as unstable.



Aliphatic index: 66.87

Grand average of hydropathicity (GRAVY): -0.636