ProtParam
User-provided sequence:
10 20 30 40 50 60
MTHLERSRHQ QHPFHMVMPS PWPIVVSFAL LSLALSTALT MHGYIGNMNM VYLALFVLLT
70 80 90 100 110 120
SSILWFRDIV AEATYLGDHT MAVRKGINLG FLMFVLSEVL IFAGLFWAYF HSAMSPDVTL
130 140 150 160 170 180
GACWPPVGIE AVQPTELPLL NTIILLSSGA TVTYSHHALI AGNRNKALSG LLITFWLIVI
190 200 210 220 230 240
FVTCQYIEYT NAAFTISDGV YGSVFYAGTG LHFLHMVMLA AMLGVNYWRM RNYHLTAGHH
250 260
VGYETTIIYT HVLDVIWLFL YVVFYWWGV
References and
documentation are available.
Number of amino acids: 269
Molecular weight: 30359.71
Theoretical pI: 6.55
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 9
Atomic composition:
Carbon C 1430
Hydrogen H 2140
Nitrogen N 344
Oxygen O 358
Sulfur S 15
Formula: C
1430H
2140N
344O
358S
15
Total number of atoms: 4287
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71975
Abs 0.1% (=1 g/l) 2.371, assuming all pairs of Cys residues form cystines
Ext. coefficient 71850
Abs 0.1% (=1 g/l) 2.367, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.19
This classifies the protein as stable.
Aliphatic index: 113.42
Grand average of hydropathicity (GRAVY): 0.690