ProtParam
User-provided sequence:
10 20 30 40 50 60
MTHPVISLKP SYNSVIRGCP GLPDTLPRIE CQLRVRSNNS LPFKLVKIEI VLKTIEIYFN
70 80 90 100 110 120
KNLYSSNNSS FTPFNRPSDP SNGHSDTSNQ NISIHYKKNI VLSHPTHDGD DLNNDLIGID
130 140 150 160 170 180
IPLTIGLPDD IKETNYNPKF GKTQTFLDCT VFYTEVGGGS SNKKRNFLYP VNVERYTYLP
190 200 210 220 230 240
SPSYFRPINR SNITSPDQKF LISYSIENPC VSMNNDTLKL SISIRLNPFP NNATTPSSND
250 260 270 280 290 300
FDVSTPTLFS TKKKFKSKLK LKSITTQILE YLEILKNQSE FSSTQTTNIL QTSVRQVDQI
310 320 330 340 350 360
ISMNSMIFQF NLKIFTKDKI LQSFRSSESS CPETKVLINK IDDIPLQYHS SITTIGQHFN
370 380 390 400 410 420
VSHYLSIRFK FNKSLKNFEI NHPLIISFWS VSQLPLIENL ILQERQTAKF AKKFYKNFGR
430 440 450 460 470
IKNTSNNNNS SNCLEYPSLP PIIYNFNDPE TNNRFNILYS QKDPSRTDPS KLRRVPVIQ
References and
documentation are available.
Number of amino acids: 479
Molecular weight: 54893.46
Theoretical pI: 9.43
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2466
Hydrogen H 3873
Nitrogen N 661
Oxygen O 737
Sulfur S 10
Formula: C
2466H
3873N
661O
737S
10
Total number of atoms: 7747
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32695
Abs 0.1% (=1 g/l) 0.596, assuming all pairs of Cys residues form cystines
Ext. coefficient 32320
Abs 0.1% (=1 g/l) 0.589, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.46
This classifies the protein as unstable.
Aliphatic index: 83.78
Grand average of hydropathicity (GRAVY): -0.490